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Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation
Qin Xiao-ying; Li Guo-xuan; Qin Ya-zhen; Wang Yu; Wang Feng-rong; Liu Dai-hong; Xu Lan-ping; Chen Huan; Han Wei; Wang Jing-zhi; Zhang Xiao-hui; Li Jin-lan; Li Ling-di; Liu Kai-yan; Huang Xiao-jun
关键词Chimerism Graft Rejection Hematopoietic Stem Cell Transplantation Polymerase Chain Reaction Single Nucleotide Polymorphism
刊名CHINESE MEDICAL JOURNAL
2012-06-05
DOI10.3760/cma.j.issn.0366-6999.2012.11.021
125期:11页:1952-1959
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]BONE-MARROW-TRANSPLANTATION ; POLYMERASE-CHAIN-REACTION ; MINIMAL RESIDUAL DISEASE ; SINGLE NUCLEOTIDE POLYMORPHISMS ; MULTIPLEX PCR AMPLIFICATION ; ACUTE MYELOID-LEUKEMIA ; TANDEM REPEAT-MARKERS ; REAL-TIME PCR ; MIXED CHIMERISM ; PERIPHERAL-BLOOD
英文摘要

Background Chimerism analysis is an important tool for the surveillance of post-transplant engraftment. It offers the possibility of identifying impending graft rejection and recurrence of underlying malignant or non-malignant disease. Here we investigated the quantitative chimerism kinetics of 21 relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation (HSCT).

Methods A panel of 29 selected sequence polymorphism (SP) markers was screened by real-time polymerase chain reaction (RT-PCR) to obtain the informative marker for every leukemia patient. Quantitative chimerism analysis of bone marrow (BM) samples of 21 relapsed patients and 20 patients in stable remission was performed longitudinally. The chimerisms of BM and peripheral blood (PB) samples of 14 patients at relapse were compared.

Results Twenty-one patients experienced leukemia relapse at a median of 135 days (range, 30-720 days) after transplantation. High recipient chimerism in BM was found in all patients at relapse, and increased recipient chimerism in BM samples was observed in 90% (19/21) of patients before relapse. With 0.5% recipient DNA as the cut-off, median time between the detection of increased recipient chimerism and relapse was 45 days (range, 0-120 days), with 76% of patients showing increased recipient chimerism at least 1 month prior to relapse. Median percentage of recipient DNA in 20 stable remission patients was 0.28%, 0.04%, 0.05%, 0.05%, 0.08%, and 0.05% at 1, 2, 3, 6, 9, and 12 months, respectively, after transplantation. This was concordant with other specific fusion transcripts and fluorescent in situ hybridization examination. The recipient chimerisms in BM were significantly higher than those in PB at relapse (P=0.001).

Conclusions This SP-based RT-PCR assay is a reliable method for chimerism analysis. Chimerism kinetics in BM can be used as a marker of impending leukemia relapse, especially when no other specific marker is available. Based on our findings, we recommend examining not only PB samples but also BM samples in HSCT patients. Chin Med J 2012;125(11):1952-1959

语种英语
WOS记录号WOS:000305259700022
项目编号30725038 ; IRT0702 ; 2006AA02A405
资助机构National Outstanding Young Scientists&prime ; Foundation of China ; Program for Innovative Research Team in University ; Hi-Tech Research and Development Program of China "863"
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54534
专题北京大学第二临床医学院_血液科
医学人文研究院/公共教学部_哲学与社会科学系
作者单位Peking Univ, Peoples Hosp, Inst Hematol, Beijing Key Lab Hematopoiet Stem Cell Transplanta, Beijing 100044, Peoples R China
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GB/T 7714
Qin Xiao-ying,Li Guo-xuan,Qin Ya-zhen,et al. Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation[J]. CHINESE MEDICAL JOURNAL,2012,125(11):1952-1959.
APA Qin Xiao-ying.,Li Guo-xuan.,Qin Ya-zhen.,Wang Yu.,Wang Feng-rong.,...&Huang Xiao-jun.(2012).Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation.CHINESE MEDICAL JOURNAL,125(11),1952-1959.
MLA Qin Xiao-ying,et al."Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation".CHINESE MEDICAL JOURNAL 125.11(2012):1952-1959.
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