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学科主题: 临床医学
题名:
Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation
作者: Qin Xiao-ying; Li Guo-xuan; Qin Ya-zhen; Wang Yu; Wang Feng-rong; Liu Dai-hong; Xu Lan-ping; Chen Huan; Han Wei; Wang Jing-zhi; Zhang Xiao-hui; Li Jin-lan; Li Ling-di; Liu Kai-yan; Huang Xiao-jun
关键词: chimerism ; graft rejection ; hematopoietic stem cell transplantation ; polymerase chain reaction ; single nucleotide polymorphism
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2012-06-05
DOI: 10.3760/cma.j.issn.0366-6999.2012.11.021
卷: 125, 期:11, 页:1952-1959
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: BONE-MARROW-TRANSPLANTATION ; POLYMERASE-CHAIN-REACTION ; MINIMAL RESIDUAL DISEASE ; SINGLE NUCLEOTIDE POLYMORPHISMS ; MULTIPLEX PCR AMPLIFICATION ; ACUTE MYELOID-LEUKEMIA ; TANDEM REPEAT-MARKERS ; REAL-TIME PCR ; MIXED CHIMERISM ; PERIPHERAL-BLOOD
英文摘要:

Background Chimerism analysis is an important tool for the surveillance of post-transplant engraftment. It offers the possibility of identifying impending graft rejection and recurrence of underlying malignant or non-malignant disease. Here we investigated the quantitative chimerism kinetics of 21 relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation (HSCT).

Methods A panel of 29 selected sequence polymorphism (SP) markers was screened by real-time polymerase chain reaction (RT-PCR) to obtain the informative marker for every leukemia patient. Quantitative chimerism analysis of bone marrow (BM) samples of 21 relapsed patients and 20 patients in stable remission was performed longitudinally. The chimerisms of BM and peripheral blood (PB) samples of 14 patients at relapse were compared.

Results Twenty-one patients experienced leukemia relapse at a median of 135 days (range, 30-720 days) after transplantation. High recipient chimerism in BM was found in all patients at relapse, and increased recipient chimerism in BM samples was observed in 90% (19/21) of patients before relapse. With 0.5% recipient DNA as the cut-off, median time between the detection of increased recipient chimerism and relapse was 45 days (range, 0-120 days), with 76% of patients showing increased recipient chimerism at least 1 month prior to relapse. Median percentage of recipient DNA in 20 stable remission patients was 0.28%, 0.04%, 0.05%, 0.05%, 0.08%, and 0.05% at 1, 2, 3, 6, 9, and 12 months, respectively, after transplantation. This was concordant with other specific fusion transcripts and fluorescent in situ hybridization examination. The recipient chimerisms in BM were significantly higher than those in PB at relapse (P=0.001).

Conclusions This SP-based RT-PCR assay is a reliable method for chimerism analysis. Chimerism kinetics in BM can be used as a marker of impending leukemia relapse, especially when no other specific marker is available. Based on our findings, we recommend examining not only PB samples but also BM samples in HSCT patients. Chin Med J 2012;125(11):1952-1959

语种: 英语
所属项目编号: 30725038 ; IRT0702 ; 2006AA02A405
项目资助者: National Outstanding Young Scientists&prime ; Foundation of China ; Program for Innovative Research Team in University ; Hi-Tech Research and Development Program of China "863"
WOS记录号: WOS:000305259700022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54534
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hematol, Beijing Key Lab Hematopoiet Stem Cell Transplanta, Beijing 100044, Peoples R China

Recommended Citation:
Qin Xiao-ying,Li Guo-xuan,Qin Ya-zhen,et al. Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation[J]. CHINESE MEDICAL JOURNAL,2012,125(11):1952-1959.
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