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学科主题临床医学
Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis
Chen, Li1,2; Tao, Yong1,2; Feng, Jing1,2; Jiang, Yan Rong1,2
刊名JOURNAL OF OPHTHALMOLOGY
2015
DOI10.1155/2015/186946
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Ophthalmology
研究领域[WOS]Research & Experimental Medicine ; Ophthalmology
关键词[WOS]SMOOTH-MUSCLE-CELLS ; DIABETIC-RETINOPATHY ; SUPPRESSES APOPTOSIS ; GLUCOSE DEPRIVATION ; SIGNALING PATHWAYS ; ANGIOGENIC FACTOR ; BLOOD-VESSELS ; MULLER CELLS ; IN-VITRO ; PROLIFERATION
英文摘要

Pericytes are a population of cells that participate in normal vessel architecture and regulate permeability. Apelin, as the endogenous ligand of G protein-coupled receptor APJ, participates in a number of physiological and pathological processes. To date, the effect of apelin on pericyte is not clear. Our study aimed to investigate the potential protection mechanisms of apelin, with regard to primary rat retinal pericytes under hypoxia. Immunofluorescence staining revealed that pericytes colocalized with APJ in the fibrovascular membranes dissected from proliferative diabetic retinopathy patients. In the in vitro studies, we first demonstrated that the expression of apelin/APJ was upregulated in pericytes under hypoxia, and apelin increased pericytes proliferation and migration. Moreover, knockdown of apelin in pericyte was achieved via lentivirus-mediated RNA interference. After the inhibition of apelin, pericytes proliferation was inhibited significantly in hypoxia culture condition. Furthermore, exogenous recombinant apelin effectively prevented hypoxia-induced apoptosis through downregulating active-caspase 3 expression and increasing the ratio of B cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) in pericytes. These results suggest that apelin suppressed hypoxia-induced pericytes injury, which indicated that apelin could be a potential therapeutic target for retinal angiogenic diseases.

语种英语
WOS记录号WOS:000362750400001
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54549
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100044, Peoples R China
2.Minist Educ, Key Lab Vis Loss & Restorat, Beijing Key Lab Diag & Therapy Retinal & Choroid, Beijing 100044, Peoples R China
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GB/T 7714
Chen, Li,Tao, Yong,Feng, Jing,et al. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis[J]. JOURNAL OF OPHTHALMOLOGY,2015.
APA Chen, Li,Tao, Yong,Feng, Jing,&Jiang, Yan Rong.(2015).Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis.JOURNAL OF OPHTHALMOLOGY.
MLA Chen, Li,et al."Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis".JOURNAL OF OPHTHALMOLOGY (2015).
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