IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells
Yang, Jing1,2; Klassen, Henry1; Pries, Mette2; Wang, Wei3; Nissen, Mogens H.2
关键词Retinal Progenitor Cells Vitreous Humor Ascorbic Acid Cell Culture
刊名JOURNAL OF NEUROSCIENCE RESEARCH
2009-02-01
DOI10.1002/jnr.21873
87期:2页:495-502
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者Danish Eye Foundation ; Novo Nordic Foundation ; Danish Eye Health Society ; Synoptic Foundation ; John and Birthe Meyer Foundation ; Discovery Eye Foundation ; Lincy Foundation ; Research to Prevent Blindness ; Danish Eye Foundation ; Novo Nordic Foundation ; Danish Eye Health Society ; Synoptic Foundation ; John and Birthe Meyer Foundation ; Discovery Eye Foundation ; Lincy Foundation ; Research to Prevent Blindness
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; PIGMENT EPITHELIAL-CELLS ; ASCORBIC-ACID ; PROGENITOR CELLS ; ANTIOXIDANT PROTECTION ; SUBRETINAL FLUID ; AQUEOUS-HUMOR ; OPTIC-NERVE ; FACTOR-BETA ; GUINEA-PIG
英文摘要

Intravitreal injection is an important delivery route for studies involving the transplantation of various types of precursor cells to the retina; however, the effect on these cells of exposure to the vitreous microenvironment has not been specifically investigated. Here vitreous humor was evaluated for the potential to influence the proliferation of rat retinal precursor cells in vitro. Cells were isolated at embryonic day 19 and plated in standard proliferation medium in the presence or absence of fluid expressed from porcine vitreous humor. Cellular proliferation at different concentrations of vitreous fluid supplementation was quantified by using a 3 H-thymidine incorporation assay. Active components of vitreous fluid were partially characterized by gel filtration chromatography (GFC) and UV spectral analysis. The effect of each vitreous fraction on proliferation was determined as well. Results showed that addition of 20% vitreous fluid to primary rat retinal cultures significantly increased 3 H-thymidine incorporation compared with growth medium without vitreous supplementation. A vitreous fraction showing growth-promoting activity was localized to a molecular mass range < 1000 Da, consistent with ascorbic acid. Ascorbic acid was confirmed in vitreous fluid by UV spectral analysis. Growth-augmenting activity was present in higher molecular mass vitreous fractions, consistent with protein components. Albumin, the major protein in vitreous fluid, was found to augment proliferation. Because vitreous-associated augmentation of retinal precursor proliferation remains an epidermal growth factor-dependent phenomenon, the proliferative status of transplanted cells in the vitreous cavity is likely determined by a combination of factors. (c) 2008 Wiley-Liss, Inc.

语种英语
资助者Danish Eye Foundation ; Novo Nordic Foundation ; Danish Eye Health Society ; Synoptic Foundation ; John and Birthe Meyer Foundation ; Discovery Eye Foundation ; Lincy Foundation ; Research to Prevent Blindness ; Danish Eye Foundation ; Novo Nordic Foundation ; Danish Eye Health Society ; Synoptic Foundation ; John and Birthe Meyer Foundation ; Discovery Eye Foundation ; Lincy Foundation ; Research to Prevent Blindness
WOS记录号WOS:000263079300020
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54561
专题北京大学第三临床医学院_眼科
作者单位1.Univ Calif Irvine, Dept Ophthalmol, Irvine, CA 92868 USA
2.Univ Copenhagen, Panum Inst, Dept Int Hlth Immunol & Microbiol, Expt Immunol Lab, DK-2200 Copenhagen, Denmark
3.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Yang, Jing,Klassen, Henry,Pries, Mette,et al. Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2009,87(2):495-502.
APA Yang, Jing,Klassen, Henry,Pries, Mette,Wang, Wei,&Nissen, Mogens H..(2009).Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells.JOURNAL OF NEUROSCIENCE RESEARCH,87(2),495-502.
MLA Yang, Jing,et al."Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells".JOURNAL OF NEUROSCIENCE RESEARCH 87.2(2009):495-502.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Jing]的文章
[Klassen, Henry]的文章
[Pries, Mette]的文章
百度学术
百度学术中相似的文章
[Yang, Jing]的文章
[Klassen, Henry]的文章
[Pries, Mette]的文章
必应学术
必应学术中相似的文章
[Yang, Jing]的文章
[Klassen, Henry]的文章
[Pries, Mette]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。