IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells
Yang, Jing1,2; Klassen, Henry1; Pries, Mette2; Wang, Wei3; Nissen, Mogens H.2
关键词Retinal Progenitor Cells Vitreous Humor Ascorbic Acid Cell Culture
刊名JOURNAL OF NEUROSCIENCE RESEARCH
2009-02-01
DOI10.1002/jnr.21873
87期:2页:495-502
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; PIGMENT EPITHELIAL-CELLS ; ASCORBIC-ACID ; PROGENITOR CELLS ; ANTIOXIDANT PROTECTION ; SUBRETINAL FLUID ; AQUEOUS-HUMOR ; OPTIC-NERVE ; FACTOR-BETA ; GUINEA-PIG
英文摘要

Intravitreal injection is an important delivery route for studies involving the transplantation of various types of precursor cells to the retina; however, the effect on these cells of exposure to the vitreous microenvironment has not been specifically investigated. Here vitreous humor was evaluated for the potential to influence the proliferation of rat retinal precursor cells in vitro. Cells were isolated at embryonic day 19 and plated in standard proliferation medium in the presence or absence of fluid expressed from porcine vitreous humor. Cellular proliferation at different concentrations of vitreous fluid supplementation was quantified by using a 3 H-thymidine incorporation assay. Active components of vitreous fluid were partially characterized by gel filtration chromatography (GFC) and UV spectral analysis. The effect of each vitreous fraction on proliferation was determined as well. Results showed that addition of 20% vitreous fluid to primary rat retinal cultures significantly increased 3 H-thymidine incorporation compared with growth medium without vitreous supplementation. A vitreous fraction showing growth-promoting activity was localized to a molecular mass range < 1000 Da, consistent with ascorbic acid. Ascorbic acid was confirmed in vitreous fluid by UV spectral analysis. Growth-augmenting activity was present in higher molecular mass vitreous fractions, consistent with protein components. Albumin, the major protein in vitreous fluid, was found to augment proliferation. Because vitreous-associated augmentation of retinal precursor proliferation remains an epidermal growth factor-dependent phenomenon, the proliferative status of transplanted cells in the vitreous cavity is likely determined by a combination of factors. (c) 2008 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000263079300020
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54561
Collection北京大学第三临床医学院_眼科
作者单位1.Univ Calif Irvine, Dept Ophthalmol, Irvine, CA 92868 USA
2.Univ Copenhagen, Panum Inst, Dept Int Hlth Immunol & Microbiol, Expt Immunol Lab, DK-2200 Copenhagen, Denmark
3.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Yang, Jing,Klassen, Henry,Pries, Mette,et al. Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2009,87(2):495-502.
APA Yang, Jing,Klassen, Henry,Pries, Mette,Wang, Wei,&Nissen, Mogens H..(2009).Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells.JOURNAL OF NEUROSCIENCE RESEARCH,87(2),495-502.
MLA Yang, Jing,et al."Vitreous Humor and Albumin Augment the Proliferation of Cultured Retinal Precursor Cells".JOURNAL OF NEUROSCIENCE RESEARCH 87.2(2009):495-502.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Yang, Jing]'s Articles
[Klassen, Henry]'s Articles
[Pries, Mette]'s Articles
百度学术
百度学术Similar articles in
[Yang, Jing]'s Articles
[Klassen, Henry]'s Articles
[Pries, Mette]'s Articles
必应学术
必应学术Similar articles in
[Yang, Jing]'s Articles
[Klassen, Henry]'s Articles
[Pries, Mette]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.