学科主题基础医学
Preparation of a Monoclonal Antibody against Notoginsenoside R1, a Distinctive Saponin from Panax notoginseng, and Its Application to Indirect Competitive ELISA
Limsuwanchote, Supattra1; Wungsintaweekul, Juraithip1; Yusakul, Gorawit3; Han, Jing-Yan2; Sasaki-Tabata, Kaori3; Tanaka, Hiroyuki3; Shoyama, Yukihiro4; Morimoto, Satoshi3
关键词Notoginsenoside R1 Elisa Monoclonal Antibody Panax Notoginseng Araliaceae Sanqi Ginseng
刊名PLANTA MEDICA
2014-03-01
DOI10.1055/s-0033-1360394
80期:4页:337-342
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]PRESSURIZED LIQUID EXTRACTION ; MAJOR GINSENG COMPONENT ; MASS-SPECTROMETRY ; HPLC-ELSD ; GINSENOSIDES ; RB1 ; RG1 ; CHROMATOGRAPHY ; HYBRIDOMAS ; ROOTS
英文摘要

We have prepared a monoclonal antibody against notoginsenoside R1, a primary active constituent of Sanqi ginseng (roots of Panax notoginseng). The monoclonal antibody was raised by immunizing BALB/c male mice with notoginsenoside R1-bovine albumin conjugates following cell fusion via electroporation. This method has been shown to be very effective for producing hybridomas with excellent antibody prevalence following cell fusion of their splenocytes with cells of the myeloma cell line SP2/0. Of all the hybridomas secreting a monoclonal antibody against notoginsenoside R1, only the 1A1P cell line produces a highly specific antibody to this compound. Surprisingly, the cross-reactivity of this monoclonal antibody for ginsenoside Rg1 and ginsenoside Re, derivatives of protopanaxatriol, was below 1.02% in a competitive immunoassay. Based on this characteristic of the monoclonal antibody, an indirect competitive ELISA (range of measurement 0.56-9 mu g/mL) was established and applied as a quality control method. In conclusion, the developed immunoassay was easy to handle and reliable for the analysis of notoginsenoside R1 in Sanqi ginseng products without requiring a pretreatment.

语种英语
WOS记录号WOS:000332395000013
项目编号PHD/0311/2550
资助机构JSPS&prime ; s Asian CORE Program ; Ministry of Education, Culture, Sports, Science, and Technology of Japan ; Kyushu University Foundation ; Royal Golden Jubilee Ph.D Program ; Thailand Research Fund
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54562
专题北京大学基础医学院_北大医学部天士力微循环研究中心
作者单位1.Nagasaki Int Univ, Dept Pharmacognosy, Sasebo, Nagasaki, Japan
2.Prince Songkla Univ, Fac Pharmaceut Sci, Hat Yai, Songkhla, Thailand
3.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100871, Peoples R China
4.Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Pharmacognosy, Fukuoka 8128582, Japan
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GB/T 7714
Limsuwanchote, Supattra,Wungsintaweekul, Juraithip,Yusakul, Gorawit,et al. Preparation of a Monoclonal Antibody against Notoginsenoside R1, a Distinctive Saponin from Panax notoginseng, and Its Application to Indirect Competitive ELISA[J]. PLANTA MEDICA,2014,80(4):337-342.
APA Limsuwanchote, Supattra.,Wungsintaweekul, Juraithip.,Yusakul, Gorawit.,Han, Jing-Yan.,Sasaki-Tabata, Kaori.,...&Morimoto, Satoshi.(2014).Preparation of a Monoclonal Antibody against Notoginsenoside R1, a Distinctive Saponin from Panax notoginseng, and Its Application to Indirect Competitive ELISA.PLANTA MEDICA,80(4),337-342.
MLA Limsuwanchote, Supattra,et al."Preparation of a Monoclonal Antibody against Notoginsenoside R1, a Distinctive Saponin from Panax notoginseng, and Its Application to Indirect Competitive ELISA".PLANTA MEDICA 80.4(2014):337-342.
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