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IR@PKUHSC  > 基础医学院  > 北大医学部天士力微循环研究中心  > 期刊论文
学科主题: 基础医学
题名:
Preparation of a Monoclonal Antibody against Notoginsenoside R1, a Distinctive Saponin from Panax notoginseng, and Its Application to Indirect Competitive ELISA
作者: Limsuwanchote, Supattra1; Wungsintaweekul, Juraithip1; Yusakul, Gorawit3; Han, Jing-Yan2; Sasaki-Tabata, Kaori3; Tanaka, Hiroyuki3; Shoyama, Yukihiro4; Morimoto, Satoshi3
关键词: notoginsenoside R1 ; ELISA ; monoclonal antibody ; Panax notoginseng ; Araliaceae ; Sanqi ginseng
刊名: PLANTA MEDICA
发表日期: 2014-03-01
DOI: 10.1055/s-0033-1360394
卷: 80, 期:4, 页:337-342
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]: PRESSURIZED LIQUID EXTRACTION ; MAJOR GINSENG COMPONENT ; MASS-SPECTROMETRY ; HPLC-ELSD ; GINSENOSIDES ; RB1 ; RG1 ; CHROMATOGRAPHY ; HYBRIDOMAS ; ROOTS
英文摘要:

We have prepared a monoclonal antibody against notoginsenoside R1, a primary active constituent of Sanqi ginseng (roots of Panax notoginseng). The monoclonal antibody was raised by immunizing BALB/c male mice with notoginsenoside R1-bovine albumin conjugates following cell fusion via electroporation. This method has been shown to be very effective for producing hybridomas with excellent antibody prevalence following cell fusion of their splenocytes with cells of the myeloma cell line SP2/0. Of all the hybridomas secreting a monoclonal antibody against notoginsenoside R1, only the 1A1P cell line produces a highly specific antibody to this compound. Surprisingly, the cross-reactivity of this monoclonal antibody for ginsenoside Rg1 and ginsenoside Re, derivatives of protopanaxatriol, was below 1.02% in a competitive immunoassay. Based on this characteristic of the monoclonal antibody, an indirect competitive ELISA (range of measurement 0.56-9 mu g/mL) was established and applied as a quality control method. In conclusion, the developed immunoassay was easy to handle and reliable for the analysis of notoginsenoside R1 in Sanqi ginseng products without requiring a pretreatment.

语种: 英语
所属项目编号: PHD/0311/2550
项目资助者: JSPS&prime ; s Asian CORE Program ; Ministry of Education, Culture, Sports, Science, and Technology of Japan ; Kyushu University Foundation ; Royal Golden Jubilee Ph.D Program ; Thailand Research Fund
WOS记录号: WOS:000332395000013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54562
Appears in Collections:基础医学院_北大医学部天士力微循环研究中心_期刊论文

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作者单位: 1.Nagasaki Int Univ, Dept Pharmacognosy, Sasebo, Nagasaki, Japan
2.Prince Songkla Univ, Fac Pharmaceut Sci, Hat Yai, Songkhla, Thailand
3.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100871, Peoples R China
4.Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Pharmacognosy, Fukuoka 8128582, Japan

Recommended Citation:
Limsuwanchote, Supattra,Wungsintaweekul, Juraithip,Yusakul, Gorawit,et al. Preparation of a Monoclonal Antibody against Notoginsenoside R1, a Distinctive Saponin from Panax notoginseng, and Its Application to Indirect Competitive ELISA[J]. PLANTA MEDICA,2014,80(4):337-342.
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