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学科主题基础医学
Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells
Yang, Yuxia1; Wang, Saifeng1; Zhang, Yanju2; Zhu, Xiaoling1
关键词RBM15 RNA interference CML apoptosis cell cycle
刊名LEUKEMIA & LYMPHOMA
2012-11-01
DOI10.3109/10428194.2012.684350
53期:11页:2237-2244
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Hematology
资助者Beijing Natural Science Foundation ; Leading Academic Discipline Project of Beijing Education Bureau ; Beijing Natural Science Foundation ; Leading Academic Discipline Project of Beijing Education Bureau
研究领域[WOS]Oncology ; Hematology
关键词[WOS]CHRONIC MYELOID-LEUKEMIA ; HEMATOPOIETIC STEM-CELL ; SPLIT-ENDS ; SIGNALING PATHWAY ; MOTIF PROTEIN ; GENE ; SUPPRESSION ; RESISTANCE ; MUTATIONS ; COMPONENT
英文摘要

RNA binding motif protein 15 (RBM15) was originally described as a 5′ translocation partner of the MAL gene in t(1;22)(p13;q13) infant acute megakaryocytic leukemia. Although previous investigations have shown that Rbm15 has broad regulatory effects within murine hematopoiesis through modulating Notch-induced transcriptional activation, which plays key roles in leukemogenesis, it is not clear what the functions of RBM15 are in the regulation of hematological malignancies. In the present study, we show that RBM15 expression was significantly increased in patients with blast-crisis chronic myelogenous leukemia (CML) compared with chronic-phase or accelerated-phase disease by real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. To further elucidate the role of RBM15 in CML, we introduced RBM15 small interfering RNA (siRNA) using pSUPER into CML cells. Fluorescence activated cell sorting (FACS), real-time RT-PCR and Western blot were used to study changes in RBM15 expression levels in transduced cells by comparing with control cells. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle and induce apoptosis in CML cells. Knockdown of RBM15 may also act to inhibit clonogenicity and induce differentiation of CML cells along the myeloid lineage. Our studies also show that the effects of RBM15 on CML cells may be mediated, at least in part, via its effect on Notch signaling. These findings demonstrate that RBM15 does indeed play a critical role in the survival of CML cells, which may have potential application in designing molecular therapies for CML treatment.

语种英语
所属项目编号5122022 ; 8910026970103004
资助者Beijing Natural Science Foundation ; Leading Academic Discipline Project of Beijing Education Bureau ; Beijing Natural Science Foundation ; Leading Academic Discipline Project of Beijing Education Bureau
WOS记录号WOS:000309475600025
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54564
专题北京大学基础医学院_医学遗传学系
作者单位1.Tianjin Cent Hosp Obstet & Gynecol, Tianjin, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Med Genet, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Yang, Yuxia,Wang, Saifeng,Zhang, Yanju,et al. Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells[J]. LEUKEMIA & LYMPHOMA,2012,53(11):2237-2244.
APA Yang, Yuxia,Wang, Saifeng,Zhang, Yanju,&Zhu, Xiaoling.(2012).Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells.LEUKEMIA & LYMPHOMA,53(11),2237-2244.
MLA Yang, Yuxia,et al."Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells".LEUKEMIA & LYMPHOMA 53.11(2012):2237-2244.
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