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学科主题: 基础医学
题名:
Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production
作者: Zhang, Hongwei1; Xie, Jun1; Xie, Qing1,2; Wilson, James M.3; Gao, Guangping1
刊名: HUMAN GENE THERAPY
发表日期: 2009-09-01
DOI: 10.1089/hum.2009.125
卷: 20, 期:9, 页:922-929
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]: HUMAN-PAPILLOMAVIRUS TYPE-16 ; PACKAGING CELL-LINES ; REP-CAP SEQUENCES ; GENE-THERAPY ; IN-VITRO ; EFFICIENT TRANSDUCTION ; COLUMN CHROMATOGRAPHY ; VECTORS ; AAV ; AMPLIFICATION
英文摘要:

Recombinant adeno-associated virus (rAAV) holds promise for applications in gene therapy. Advances in clinical studies of rAAV-based gene therapeutics have generated an encouraging momentum in the field of gene therapy; however, one of the major obstacles to the eventual clinical success of rAAV-mediated gene therapy is the need for large-scale production of clinical-grade vectors. The transfection-based rAAV production method is well suited for preclinical studies in small animal models, but it is difficult to support large-scale clinical studies with this method. In the past decade, several scalable rAAV production methods have emerged from extensive efforts to develop large-scale manufacturing processes. Among those, the recombinant adenovirus-AAV infection method has some unique features in vector quality and yield. This minireview provides an overview of this scaleable rAAV production platform, describing its basic components and biological mechanisms and process.

语种: 英语
WOS记录号: WOS:000269673100002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54571
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA 01605 USA
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
3.Univ Penn, Sch Med, Gene Therapy Program, Philadelphia, PA 19104 USA

Recommended Citation:
Zhang, Hongwei,Xie, Jun,Xie, Qing,et al. Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production[J]. HUMAN GENE THERAPY,2009,20(9):922-929.
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