IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production
Zhang, Hongwei1; Xie, Jun1; Xie, Qing1,2; Wilson, James M.3; Gao, Guangping1
刊名HUMAN GENE THERAPY
2009-09-01
DOI10.1089/hum.2009.125
20期:9页:922-929
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]HUMAN-PAPILLOMAVIRUS TYPE-16 ; PACKAGING CELL-LINES ; REP-CAP SEQUENCES ; GENE-THERAPY ; IN-VITRO ; EFFICIENT TRANSDUCTION ; COLUMN CHROMATOGRAPHY ; VECTORS ; AAV ; AMPLIFICATION
英文摘要

Recombinant adeno-associated virus (rAAV) holds promise for applications in gene therapy. Advances in clinical studies of rAAV-based gene therapeutics have generated an encouraging momentum in the field of gene therapy; however, one of the major obstacles to the eventual clinical success of rAAV-mediated gene therapy is the need for large-scale production of clinical-grade vectors. The transfection-based rAAV production method is well suited for preclinical studies in small animal models, but it is difficult to support large-scale clinical studies with this method. In the past decade, several scalable rAAV production methods have emerged from extensive efforts to develop large-scale manufacturing processes. Among those, the recombinant adenovirus-AAV infection method has some unique features in vector quality and yield. This minireview provides an overview of this scaleable rAAV production platform, describing its basic components and biological mechanisms and process.

语种英语
WOS记录号WOS:000269673100002
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54571
Collection北京大学基础医学院_病原生物学系
作者单位1.Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA 01605 USA
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
3.Univ Penn, Sch Med, Gene Therapy Program, Philadelphia, PA 19104 USA
Recommended Citation
GB/T 7714
Zhang, Hongwei,Xie, Jun,Xie, Qing,et al. Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production[J]. HUMAN GENE THERAPY,2009,20(9):922-929.
APA Zhang, Hongwei,Xie, Jun,Xie, Qing,Wilson, James M.,&Gao, Guangping.(2009).Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production.HUMAN GENE THERAPY,20(9),922-929.
MLA Zhang, Hongwei,et al."Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production".HUMAN GENE THERAPY 20.9(2009):922-929.
Files in This Item: Download All
File Name/Size DocType Version Access License
Adenovirus-Adeno-Ass(244KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Zhang, Hongwei]'s Articles
[Xie, Jun]'s Articles
[Xie, Qing]'s Articles
百度学术
百度学术Similar articles in
[Zhang, Hongwei]'s Articles
[Xie, Jun]'s Articles
[Xie, Qing]'s Articles
必应学术
必应学术Similar articles in
[Zhang, Hongwei]'s Articles
[Xie, Jun]'s Articles
[Xie, Qing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.