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学科主题基础医学
Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production
Zhang, Hongwei1; Xie, Jun1; Xie, Qing1,2; Wilson, James M.3; Gao, Guangping1
刊名HUMAN GENE THERAPY
2009-09-01
DOI10.1089/hum.2009.125
20期:9页:922-929
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]HUMAN-PAPILLOMAVIRUS TYPE-16 ; PACKAGING CELL-LINES ; REP-CAP SEQUENCES ; GENE-THERAPY ; IN-VITRO ; EFFICIENT TRANSDUCTION ; COLUMN CHROMATOGRAPHY ; VECTORS ; AAV ; AMPLIFICATION
英文摘要

Recombinant adeno-associated virus (rAAV) holds promise for applications in gene therapy. Advances in clinical studies of rAAV-based gene therapeutics have generated an encouraging momentum in the field of gene therapy; however, one of the major obstacles to the eventual clinical success of rAAV-mediated gene therapy is the need for large-scale production of clinical-grade vectors. The transfection-based rAAV production method is well suited for preclinical studies in small animal models, but it is difficult to support large-scale clinical studies with this method. In the past decade, several scalable rAAV production methods have emerged from extensive efforts to develop large-scale manufacturing processes. Among those, the recombinant adenovirus-AAV infection method has some unique features in vector quality and yield. This minireview provides an overview of this scaleable rAAV production platform, describing its basic components and biological mechanisms and process.

语种英语
WOS记录号WOS:000269673100002
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被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54571
专题北京大学基础医学院_病原生物学系
北京大学临床肿瘤学院_核医学科
作者单位1.Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA 01605 USA
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
3.Univ Penn, Sch Med, Gene Therapy Program, Philadelphia, PA 19104 USA
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GB/T 7714
Zhang, Hongwei,Xie, Jun,Xie, Qing,et al. Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production[J]. HUMAN GENE THERAPY,2009,20(9):922-929.
APA Zhang, Hongwei,Xie, Jun,Xie, Qing,Wilson, James M.,&Gao, Guangping.(2009).Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production.HUMAN GENE THERAPY,20(9),922-929.
MLA Zhang, Hongwei,et al."Adenovirus-Adeno-Associated Virus Hybrid for Large-Scale Recombinant Adeno-Associated Virus Production".HUMAN GENE THERAPY 20.9(2009):922-929.
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