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学科主题: 临床医学
题名:
An ultrastructural study of Trichophyton rubrum induced onychomycosis
作者: Yue, Xueping1; Li, Qing1; Wang, Hongwei1; Sun, Yilin2; Wang, Aiping3; Zhang, Qi2; Zhang, Cuiping2
关键词: Onychomycosis ; Trichophyton rubrum ; Scanning electron microscopy ; Transmission electron microscopy ; Ultrastructure
刊名: BMC INFECTIOUS DISEASES
发表日期: 2015-11-17
DOI: 10.1186/s12879-015-1240-1
卷: 15
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Infectious Diseases
研究领域[WOS]: Infectious Diseases
关键词[WOS]: MENTAGROPHYTES ; GROWTH
英文摘要:

Background: Trichophyton rubrum (T. rubrum) caused onychomycosis is the most common nail fungal disease. The common diagnostic methods are direct microscopic examination and fungal culture. In this study we used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to study the subungual ultrastructural changes in T. rubrum induced onychomycosis.

Methods: Six outpatients with onychomycosis were recruited and T. rubrum infection was confirmed by fungal culture. Six toenail samples were collected and prepared for SEM characterization. The cultured fugal colonies were prepared for SEM and TEM characterization.

Results: 1) SEM showed significant structural damages and the formation of a thin layer or a single layer of keratinocytes in all infected nail plates. Hyphae (piercing or penetrating keratinocytes layers), arthrospores and local bacterial aggregation were observed on the ventral surface of the nail plates. 2) SEM of the cultured fungal colony showed relatively straight, highly branched hyphae and microconidias; TEM showed branching hyphae that were composed of double-layer cell walls. Hyphae had nucleus, mitochondria, liposomes, lysosomes, scattered rough endoplasmic reticulum, myeloid bodies and aggregated ribosomes. There were high-density particles outside the hyphae.

Conclusion: SEM showed a large number of hyphae penetrated the keratinocytes layer, suggesting that T. rubrum can cause severe damage to the stratum corneum. TEM showed the ultrastructural features of T. rubrum-induced infection before treatment.

语种: 英语
WOS记录号: WOS:000365287400003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54588
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Tiantan Hosp, Dept Dermatol & Venerol, Beijing 100050, Peoples R China
2.Capital Med Univ, Dept Ultrastruct Pathol, Beijing Neurosurg Inst, Beijing 100050, Peoples R China
3.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China

Recommended Citation:
Yue, Xueping,Li, Qing,Wang, Hongwei,et al. An ultrastructural study of Trichophyton rubrum induced onychomycosis[J]. BMC INFECTIOUS DISEASES,2015,15.
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