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Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot
Wang, YD; Li, Y; Xu, GB; Dong, XY; Yang, XA; Feng, ZR; Tian, C; Chen, WF
关键词SARS ELISA WB antibody immune response
刊名CLINICAL IMMUNOLOGY
2004-11-01
DOI10.1016/j.clim.2004.07.003
113期:2页:145-150
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]CORONAVIRUS SPIKE PROTEIN ; ACUTE RESPIRATORY SYNDROME ; AMINOPEPTIDASE-N CD13 ; RECEPTOR-BINDING ; FUNCTIONAL-CHARACTERIZATION ; FUSION PROTEIN ; AMINO-ACIDS ; DETERMINANTS ; GLYCOPROTEIN ; EXPRESSION
英文摘要

Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases (n=20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-Cov. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1-75%, S1-N-25%, S1-C-55%). Evaluation of sera from healthy controls (n=60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals (n=10) with SARS-like symptoms (ELISA-100% positive, S2 WB-30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-neqative SARS cases and in some ELISA-positive non-SARS cases. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000224333400006
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54594
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Dept Lab Examinat, Beijing 100871, Peoples R China
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GB/T 7714
Wang, YD,Li, Y,Xu, GB,et al. Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot[J]. CLINICAL IMMUNOLOGY,2004,113(2):145-150.
APA Wang, YD.,Li, Y.,Xu, GB.,Dong, XY.,Yang, XA.,...&Chen, WF.(2004).Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot.CLINICAL IMMUNOLOGY,113(2),145-150.
MLA Wang, YD,et al."Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot".CLINICAL IMMUNOLOGY 113.2(2004):145-150.
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