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学科主题: 基础医学
题名:
Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot
作者: Wang, YD; Li, Y; Xu, GB; Dong, XY; Yang, XA; Feng, ZR; Tian, C; Chen, WF
关键词: SARS ; ELISA ; WB ; antibody ; immune response
刊名: CLINICAL IMMUNOLOGY
发表日期: 2004-11-01
DOI: 10.1016/j.clim.2004.07.003
卷: 113, 期:2, 页:145-150
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: CORONAVIRUS SPIKE PROTEIN ; ACUTE RESPIRATORY SYNDROME ; AMINOPEPTIDASE-N CD13 ; RECEPTOR-BINDING ; FUNCTIONAL-CHARACTERIZATION ; FUSION PROTEIN ; AMINO-ACIDS ; DETERMINANTS ; GLYCOPROTEIN ; EXPRESSION
英文摘要:

Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases (n=20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-Cov. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1-75%, S1-N-25%, S1-C-55%). Evaluation of sera from healthy controls (n=60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals (n=10) with SARS-like symptoms (ELISA-100% positive, S2 WB-30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-neqative SARS cases and in some ELISA-positive non-SARS cases. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000224333400006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54594
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Dept Lab Examinat, Beijing 100871, Peoples R China

Recommended Citation:
Wang, YD,Li, Y,Xu, GB,et al. Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot[J]. CLINICAL IMMUNOLOGY,2004,113(2):145-150.
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