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学科主题: 基础医学
题名:
The Role of Transcription Factors Sp1 and YY1 in Proximal Promoter Region in Initiation of Transcription of the Mu Opioid Receptor Gene in Human Lymphocytes
作者: Li, Hui1; Liu, Han1; Wang, Zimei1; Liu, Xinhua1; Guo, Liyuan1; Huang, Lijun1; Gao, Libo1; McNutt, Michael A.3; Li, Gang1,2
关键词: mu opioid receptor ; lymphocytes ; expression ; transcription factors ; morphine ; promoter
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2008-05-01
DOI: 10.1002/jcb.21616
卷: 104, 期:1, 页:237-250
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: OPIATE RECEPTOR ; NEURONAL CELLS ; IMMUNE CELLS ; EXPRESSION ; APOPTOSIS ; SEQUENCE ; CLONING ; MECHANISMS ; DIVERSITY ; CCR5
英文摘要:

Although previous studies have shown that the mechanism of the lymphocyte mu opioid receptor (MOR) gene expression was distinctly different from that in the central system, and is involved in several disparate, aspects of the immune response, its precise molecular mechanism is still undefined. In this study, we analyzed the proximal promoter region of the MOR gene in lymphocytes to identify the influences of potential trans-acting factors in activating the initiation of the expression of the MOR gene in lymphocytes. The electrophoretic mobility shift assay showed that two transcription factors, Sp1 and YY1, were able to bind the promoter region. Using sequence overlapping probes and mutation assays, we determined that the CCC secluence of Sp1 and the GGC sequence of YY1 binding elements were core sequences, and replacement of these sequences lead to substantial loss of promoter activity. Stimulation with morphine was capable of up-regulating the intracellular level of Sp1 and YY1 proteins. Chromatin immunoprecipitation assays showed that the blockage of naloxone is achieved through down-regulation of transcription factor YY1. Furthermore, coimmunoprecipitation and transfection assays confirmed that the functional interaction of Sp1 and YY1 transcription factors was a crucial step in the initiation of expression of the MOR in lymphocytes. Thus, We conclude that the cooperative interaction of Sp1 and YY1 transcription factors is the critical event triggering the initiation of transcription of the MOR gene in lymphocytes, and this finding will be helpful to understand the pharmacological effect of morphine on lymphocytes. J. Cell. Biochem. 104: 237-250, 2008. (C) 2007 Wiley-Liss, Inc.

语种: 英语
所属项目编号: 30671856 ; 30772536 ; 20030001028
项目资助者: National Natural Science Foundation of China ; Foundation of National Education Ministry
WOS记录号: WOS:000260277000021
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54611
Appears in Collections:基础医学院_北京大学感染病研究中心_期刊论文

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作者单位: 1.Peking Univ, Ctr Infect Dis, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Li, Hui,Liu, Han,Wang, Zimei,et al. The Role of Transcription Factors Sp1 and YY1 in Proximal Promoter Region in Initiation of Transcription of the Mu Opioid Receptor Gene in Human Lymphocytes[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2008,104(1):237-250.
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