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学科主题: 药学
题名:
Analysis of bufadienolides in the Chinese drug ChanSu by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry
作者: Ye, M; Guo, DA
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2005
DOI: 10.1002/rcm.1989
卷: 19, 期:13, 页:1881-1892
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Chemistry ; Spectroscopy
关键词[WOS]: CINOBUFAGIN ; BUFALIN ; FLAVONOIDS ; LC/MS/MS ; MEDICINE ; STEROIDS ; PLANT ; CELLS ; HPLC
英文摘要:

The qualitative analysis of bufadienolides in the Chinese drug ChanSu was performed using high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (APCI-MS/MS). Bufadienolides are the major bioactive constituents of ChanSu, which is used to treat heart failure and cancer in traditional Chinese medicine. The APCI-MS fragmentation behavior of bufadienolides was studied. For bufadienolides with only hydroxyl substituents, the fragmentation was characterized by successive eliminations of H2O and CO molecules, and the profile of MS/MS product ions was correlated with the number of hydroxyl groups. If a: C-16 acetoxyl group was present, the fragmentation of [M+H](+) ions was triggered by initial loss of 60 Da (HOAc). The elimination of CO was significant for bufadienolides with a 19-formyl group, and the 19-hydroxyl group could be characterized by the loss of 30 Da (HCHO). These fragmentation rules were applied to the identification of bufadienolides in a methanolic extract of ChanSu, which was separated on a C-18 column with gradient elution. A total of 35 bufadienolides were identified, including four new constituents. The method established here facilitated the convenient and rapid quality control of ChanSu crude drug and its pharmaceutical preparations. Copyright (c) 2005 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000230203500015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54622
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Ye, M,Guo, DA. Analysis of bufadienolides in the Chinese drug ChanSu by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2005,19(13):1881-1892.
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