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学科主题: 口腔医学
题名:
Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells
作者: Xiao, E.1; Yang, H. Q.4; Gan, Ye-Hua2; Duan, Deng-Hui3; He, Lin-Hai1; Guo, Yunbo4; Wang, S. Q.4; Zhang, Yi1
关键词: Marrow stromal stem cells ; Mesenchymal stem cells ; Calcium flux ; Differentiation and mechanosensation
刊名: STEM CELLS
发表日期: 2015-02-01
DOI: 10.1002/stem.1858
卷: 33, 期:2, 页:615-621
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: OSTEOBLAST DIFFERENTIATION ; FLUID-FLOW ; CHANNELS ; ACTIVATION ; KINASE ; GENE ; MECHANOSENSATION ; PROLIFERATION ; STRETCH
英文摘要:

Mesenchymal stem cells (MSCs) are multipotential stem cells residing in the bone marrow. Several studies have shown that mechanical stimulation modulates MSC differentiation through mobilization of second messengers, but the mechanism of mechanotransduction remains poorly understood. In this study, using fluorescence and laser confocal microcopy as well as patch-clamp techniques, we identified the transient receptor potential melastatin type 7 (TRPM7) channel as the key channel involved in mechanotransduction in bone marrow MSCs. TRPM7 knockdown completely abolished the pressure-induced cytosolic Ca2+ increase and pressure-induced osteogenesis. TRPM7 directly sensed membrane tension, independent of the cytoplasm and the integrity of cytoskeleton. Ca2+ influx through TRPM7 further triggered Ca2+ release from the inositol trisphosphate receptor type 2 on the endoplasmic reticulum and promoted NFATc1 nuclear localization and osteogenesis. These results identified a central role of TRPM7 in MSC mechanical stimulation-induced osteogenesis.

语种: 英语
所属项目编号: 81170936 ; 81370203 ; 2011CB809101
项目资助者: National Natural Science Foundation of China ; 973 Major State Basic Research Development Program of China
WOS记录号: WOS:000348211300026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54623
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent, Beijing 100081, Peoples R China
4.Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100081, Peoples R China

Recommended Citation:
Xiao, E.,Yang, H. Q.,Gan, Ye-Hua,et al. Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells[J]. STEM CELLS,2015,33(2):615-621.
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