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学科主题: 临床医学
题名:
Mitochondrially localized EGFR is subjected to autophagic regulation and implicated in cell survival
作者: Yue, Xiaojing2,3; Song, Weidong1; Zhang, Wei2,3; Chen, Liang1; Xi, Zhijun4; Xin, Zhongcheng1; Jiang, Xuejun2
关键词: EGFR ; mitochondria ; autophagy ; Beclin 1 ; programmed cell death
刊名: AUTOPHAGY
发表日期: 2008-07-01
卷: 4, 期:5, 页:641-649
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: EPIDERMAL-GROWTH-FACTOR ; RECEPTOR TYROSINE KINASES ; MAMMALIAN HOMOLOG ; CYTOCHROME-C ; PROTEIN ; ENDOCYTOSIS ; APOPTOSIS ; RAPAMYCIN ; DEATH ; PHOSPHORYLATION
英文摘要:

Although generally acknowledged as a plasma membrane protein, the epidermal growth factor (EGF) receptor has been found in the nucleus and subcellular organelles. Recently, the mitochondrial localization of the EGF receptor (EGFR) was reported; nevertheless, the molecular mechanism underlying EGFR localization in mitochondria is largely unknown. Using immunofluorescence and immunoelectron microscopy, we observed that EGFR did localize within mitochondria. Moreover, EGFR mitochondrial translocation can be increased by rapamycin treatment in A431 cells and greatly reduced by the presence of 3-methyladenine (3-MA), an inhibitor of autophagy. The reduction of mitochondrial EGFR via autophagy inhibition is further confirmed by small interference RNA (siRNA), through which the essential protein Beclin 1 was depleted. Knocking down Beclin I markedly decreased the mitochondrial translocation of EGFR that was induced by rapamycin. We also noticed that the content of mitochondrial EGFR transfer is decreased when the cells are exposed to the apoptotic inducer etoposide. Additionally, either EGF treatment or EGFR knockdown by siRNA results in a greater decline of cell viability in cells possessing more mitochondrial EGFRs. Taken together, we conclude that EGFR mitochondrial localization is regulated by either autophagy or programmed cell death and is correlated with cell survival.

语种: 英语
WOS记录号: WOS:000257596600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54626
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Inst Urol, Beijing 100009, Peoples R China
2.Peking Univ, Hosp 1, Androl Ctr, Beijing 100009, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China

Recommended Citation:
Yue, Xiaojing,Song, Weidong,Zhang, Wei,et al. Mitochondrially localized EGFR is subjected to autophagic regulation and implicated in cell survival[J]. AUTOPHAGY,2008,4(5):641-649.
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