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学科主题: 药学
题名:
Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MSn
作者: Zhang, Ya-Zhou1,2; Xu, Feng3; Yi, Tao1; Zhang, Jian-Ye1; Xu, Jun1; Tang, Yi-Na1; He, Xi-Chen1; Liu, Jing1; Chen, Hu-Biao1
关键词: Radix Hedysari ; Radix Astragali ; Danggui Buxue Tang ; HPLC-DAD-ESI-IT-TOF-MSn ; isoflavonoid ; astragaloside
刊名: MOLECULES
发表日期: 2014-05-01
DOI: 10.3390/molecules19055650
卷: 19, 期:5, 页:5650-5673
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Organic
研究领域[WOS]: Chemistry
关键词[WOS]: CHINESE HERBAL DECOCTION ; FLIGHT MASS-SPECTROMETRY ; RADIX ANGELICAE SINENSIS ; SIGNAL-REGULATED KINASE ; ASTRAGALI RADIX ; QUANTITATIVE-ANALYSIS ; RAT PLASMA ; IN-VIVO ; IDENTIFICATION ; METABOLITES
英文摘要:

Danggui Buxue Tang (DBT) is a Traditional Chinese Medicine (TCM) formula primarily used to treat symptoms associated with menopause in women. Usually, DBT is composed of one portion of Radix Angelicae Sinensis (RAS) and five portions of Radix Astragali (RA). Clinically, Radix Hedysari (RH) is sometimes used by TCM physicians to replace RA in DBT. In order to verity whether the chemical constituents of the DBT1 (RA:RAS = 5:1, w/w) and DBT2 (RH:RAS = 5:1, w/w) share similarities the chemical profiles of the two DBTs crude extracts and urine samples were analyzed and compared with the aid of HPLC-DAD-ESI-IT-TOF-MSn, which determines the total ion chromatogram (TIC) and multi-stage mass spectra (MSn). Then, the DBT1 and DBT2 were identified and compared on the basis of the TIC and the MSn. In the first experiment (with crude extracts), 69 compounds (C1-C69) were identified from the DBT1; 46 compounds (c1-c46) were identified from the DBT2. In the second experiment(with urine samples), 44 compounds (M1-M44) were identified from the urine samples of rats that had been administered DBT1, and 34 compounds (m1-m34) were identified from the urine samples of rats that had been administered DBT2. Identification and comparison of the chemical compositions were carried out between the DBT1 and DBT2 of the crude extracts and urine samples respectively. Our results showed that the two crude extracts of the DBTs have quite different chemical profiles. The reasons for their differences were that the special astragalosides in DBT1 and the isoflavonoid glycosides formed the malonic acid esters undergo single esterification and acetyl esters undergo acetylation in DBT1. In contrast, the urine from DBT1-treated rats strongly resembled that of DBT2-treated rats. These metabolites originate mainly from formononetin, calycosin and their related glycosides, and they were formed mainly by the metabolic process of reduction, deglycosylation, demethylation, hydrogenation and sulfation. The HPLC-DAD-ESI-IT-TOF-MSn method was successfully applied for the rapid chemical profiles evaluation of two DBTs and their related urine samples.

语种: 英语
所属项目编号: FRG2/12-13/058
项目资助者: Hong Kong Baptist University Faculty Research Foundation
WOS记录号: WOS:000337113000014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54633
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Hong Kong Baptist Univ, Baptist Univ Rd, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
2.Guizhou Coll Technol, Guiyang 550003, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Ya-Zhou,Xu, Feng,Yi, Tao,et al. Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MSn[J]. MOLECULES,2014,19(5):5650-5673.
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