|HMGN5 Knockdown Sensitizes Prostate Cancer Cells to Ionizing Radiation|
|Su, Boxing1,2,3; Shi, Bentao4; Tang, Yuan1,2,3; Guo, Zhongqiang1,2,3; Yu, Xi1,2,3; He, Xinyong5; Li, Xuesong1,2,3; Gao, Xianshu5; Zhou, Liqun1,2,3|
|关键词||Prostate Cancer High Mobility Group n 5 (Hmgn5) Ionizing Radiation|
|WOS标题词||Science & Technology|
|类目[WOS]||Endocrinology & Metabolism ; Urology & Nephrology|
|研究领域[WOS]||Endocrinology & Metabolism ; Urology & Nephrology|
|关键词[WOS]||CHROMOSOMAL-PROTEIN HMGN1 ; MOBILITY-GROUP PROTEINS ; BCL-2 FAMILY PROTEINS ; DOUBLE-STRAND BREAKS ; REACTIVE OXYGEN ; GENE-EXPRESSION ; DNA-REPAIR ; KAPPA-B ; FUTURE-DIRECTIONS ; INDUCED APOPTOSIS|
BACKGROUNDHigh Mobility Group N (HMGN) proteins are a family of chromatin structural proteins that specifically bind to nucleosome core particles. HMGN5 is a novel and characteristic member of the HMGN protein family. We have previously found that HMGN5 is upregulated in prostate cancer and its downregulation had been demonstrated to induce apoptosis and G2-M cell cycle arrest.
METHODSThe radiosensitization effect of HMGN5 knockdown on PC3 and DU145 cells was assessed using clonogenic assay, flow cytometry, and comet assay. The DNA double-strand break (DSB) repair kinetics of HMGN5 knockdown and control cells after radiation exposure was evaluated using immunocytofluorescence. The mitochondrial reactive oxygen species (ROS) levels were estimated using Dihydrorhodamine 123 (DHR 123) probes. Expression of mitochondrial antioxidant MnSOD was measured by real-time PCR and Western blot. The expression of antiapoptotic proteins Bcl-2 and Bcl-xL as well as cleavage of caspase-3, caspase-9, and PARP were also measured using Western blot.
RESULTSHMGN5 knockdown cells exhibit decreased clonogenic survival and increased apoptosis rate in response to 2-8 Gy ionizing radiation (IR). Loss of HMGN5 does not affect the DSB repair kinetics after radiation exposure. HMGN5 knockdown cells demonstrated increased mitochondrial ROS level and suppressed induction of MnSOD upon radiation compared with control cells upon radiation. Further, MnSOD knockdown resulted in inhibited cell viability as well as increased mitochondrial ROS level and apoptosis upon radiation in PC3 and DU145 cells. Finally, HMGN5 knockdown cells showed significantly decreased levels of antiapoptotic proteins Bcl-2 and Bcl-xL as well as increased cleavage of caspase-3, caspase-9, and PARP compared with control cells after radiation.
CONCLUSIONSHMGN5 knockdown sensitizes prostate cancer cells to ionizing radiation, and the radiosensitization effect may be partially mediated through suppressed induction of MnSOD and enhanced activation of apoptosis pathway in response to IR. Prostate 75:33-44, 2015. (c) 2014 Wiley Periodicals, Inc.
|项目编号||7122183 ; 81372746|
|资助机构||Beijing Natural Science Foundation ; National Natural Science Foundation|
|作者单位||1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China|
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Natl Urol Canc Ctr, Beijing, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Guangdong, Peoples R China
5.Peking Univ, Hosp 1, Dept Radiat Oncol, Beijing 100034, Peoples R China
|Su, Boxing,Shi, Bentao,Tang, Yuan,et al. HMGN5 Knockdown Sensitizes Prostate Cancer Cells to Ionizing Radiation[J]. PROSTATE,2015,75(1):33-44.|
|APA||Su, Boxing.,Shi, Bentao.,Tang, Yuan.,Guo, Zhongqiang.,Yu, Xi.,...&Zhou, Liqun.(2015).HMGN5 Knockdown Sensitizes Prostate Cancer Cells to Ionizing Radiation.PROSTATE,75(1),33-44.|
|MLA||Su, Boxing,et al."HMGN5 Knockdown Sensitizes Prostate Cancer Cells to Ionizing Radiation".PROSTATE 75.1(2015):33-44.|