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学科主题: 临床医学
题名:
Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis
作者: Zhou, Yongcui1,2; Qin, Danian2; Tang, Aifa1; Zhou, Derong1,2; Qin, Jie1; Yan, Bin1; Diao, Ruiying1; Jiang, Zhimao1; Cai, Zhiming1; Gui, Yaoting1
关键词: spermatogenesis ; azoospermia ; TSG23 ; Tsg23 ; testis
刊名: MOLECULAR HUMAN REPRODUCTION
发表日期: 2009-04-01
DOI: 10.1093/molehr/gap015
卷: 15, 期:4, 页:223-230
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: TESTIS-SPECIFIC GENE ; MOUSE SPERMATOGENESIS ; MALE-INFERTILITY ; SEMINIFEROUS EPITHELIUM ; IDENTIFICATION ; MICE ; CELLS ; CYCLE
英文摘要:

A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was located in spermatocytes and round spermatids of the seminiferous tubules in human and mouse testis. To further confirm the result from RT-PCR, the antibody for human TSG23 was generated against the protein encoded by the gene. Western blot analysis demonstrated that TSG23 was mainly expressed in human testis, with a molecular weight of about 23 kDa. Immunohistochemistry showed that TSG23 was predominantly located in spermatocytes and round spermatids, consistent with the results from in situ hybridization. In order to explore the function of TSG23 in spermatogenesis, the study compared the expression of TSG23 in the testis from fertile persons and from patients with azoospermia. The results showed that there was less expression in patients with obstructive azoospermia compared with fertile persons, and no detectable TSG23 at mRNA and protein levels in patients with non-obstructive azoospermia. The expression of Tsg23 mRNA was considerably decreased in a time-dependent manner in the testis of an azoospermic mouse model induced by Busulfan. These data suggest that TSG23/Tsg23 is involved in human and mouse spermatogenesis.

语种: 英语
所属项目编号: 30770810 ; 30700824 ; 2007B060401061 ; 200800010106 ; 2008CB517412
项目资助者: National Natural Science Foundation of China ; Science Foundation of Guangdong Province ; Chinese Foundation of Doctoral Discipline ; &prime ; 973&prime ; Project
WOS记录号: WOS:000264394600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54649
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
2.Shantou Univ, Coll Med, Dept Physiol, Shantou 515041, Peoples R China

Recommended Citation:
Zhou, Yongcui,Qin, Danian,Tang, Aifa,et al. Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis[J]. MOLECULAR HUMAN REPRODUCTION,2009,15(4):223-230.
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