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学科主题临床医学
Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis
Zhou, Yongcui1,2; Qin, Danian2; Tang, Aifa1; Zhou, Derong1,2; Qin, Jie1; Yan, Bin1; Diao, Ruiying1; Jiang, Zhimao1; Cai, Zhiming1; Gui, Yaoting1
关键词Spermatogenesis Azoospermia Tsg23 Tsg23 Testis
刊名MOLECULAR HUMAN REPRODUCTION
2009-04-01
DOI10.1093/molehr/gap015
15期:4页:223-230
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]TESTIS-SPECIFIC GENE ; MOUSE SPERMATOGENESIS ; MALE-INFERTILITY ; SEMINIFEROUS EPITHELIUM ; IDENTIFICATION ; MICE ; CELLS ; CYCLE
英文摘要

A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was located in spermatocytes and round spermatids of the seminiferous tubules in human and mouse testis. To further confirm the result from RT-PCR, the antibody for human TSG23 was generated against the protein encoded by the gene. Western blot analysis demonstrated that TSG23 was mainly expressed in human testis, with a molecular weight of about 23 kDa. Immunohistochemistry showed that TSG23 was predominantly located in spermatocytes and round spermatids, consistent with the results from in situ hybridization. In order to explore the function of TSG23 in spermatogenesis, the study compared the expression of TSG23 in the testis from fertile persons and from patients with azoospermia. The results showed that there was less expression in patients with obstructive azoospermia compared with fertile persons, and no detectable TSG23 at mRNA and protein levels in patients with non-obstructive azoospermia. The expression of Tsg23 mRNA was considerably decreased in a time-dependent manner in the testis of an azoospermic mouse model induced by Busulfan. These data suggest that TSG23/Tsg23 is involved in human and mouse spermatogenesis.

语种英语
WOS记录号WOS:000264394600003
项目编号30770810 ; 30700824 ; 2007B060401061 ; 200800010106 ; 2008CB517412
资助机构National Natural Science Foundation of China ; Science Foundation of Guangdong Province ; Chinese Foundation of Doctoral Discipline ; &prime ; 973&prime ; Project
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54649
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
2.Shantou Univ, Coll Med, Dept Physiol, Shantou 515041, Peoples R China
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GB/T 7714
Zhou, Yongcui,Qin, Danian,Tang, Aifa,et al. Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis[J]. MOLECULAR HUMAN REPRODUCTION,2009,15(4):223-230.
APA Zhou, Yongcui.,Qin, Danian.,Tang, Aifa.,Zhou, Derong.,Qin, Jie.,...&Gui, Yaoting.(2009).Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis.MOLECULAR HUMAN REPRODUCTION,15(4),223-230.
MLA Zhou, Yongcui,et al."Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis".MOLECULAR HUMAN REPRODUCTION 15.4(2009):223-230.
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