北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学公共卫生学院  > 毒理学系  > 期刊论文
学科主题: 公共卫生
题名:
In vitro studies on dissolved substance of cinnabar: Chemical species and biological properties
作者: Zhou, Xinrui2,3; Zeng, Kewu2,3; Wang, Qi1; Yang, Xiaoda2,3; Wang, Kui2,3
关键词: Cinnabar ; Mercuric polysulfide ; Caco-2 monolayer ; Protein binding
刊名: JOURNAL OF ETHNOPHARMACOLOGY
发表日期: 2010-08-19
DOI: 10.1016/j.jep.2010.06.018
卷: 131, 期:1, 页:196-202
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: ATOMIC FLUORESCENCE SPECTROMETRY ; TRADITIONAL MEDICINES ; MERCURIAL COMPOUNDS ; PROXIMAL TUBULE ; CELL-CULTURE ; ABSORPTION ; SULFIDE ; PERMEABILITY ; MONOLAYERS ; SPECIATION
英文摘要:

Cinnabar is one of traditional Chinese medicines widely used in many Asian countries. It is also a medicine with potential toxicity especially when taking overdose. Up to date, studies on the mechanism of the biological activity of cinnabar were still insufficient.

Aim of the study: To investigate the possible bioactive species from cinnabar after oral administration, which is the fundamental of biological effects of cinnabar.

Materials and methods: Under mimetic intestinal and gastric conditions, the chemical components dissolved from cinnabar were analyzed by infrared spectroscopy (IR) and Raman spectroscopy. Furthermore, binding of mercuric species of cinnabar extractions to human serum protein (HSA) was characterized and their intestinal permeability was determined using the Caco-2 cell monolayer. The cytotoxicity of cinnabar extractions was assessed on human kidney-2 (HK-2) cell.

Results: Major dissolved species included mercuric polysulfide (i.e. HgS(2)(OH)(-) and Hg(3)S(2)Cl(2)). The apparent permeability coefficient (P(app)) of mercuric polysulfides was (1.6 +/- 0.3) x 10(-6) cm/s, which is slightly lower than that of mercuric chloride (HgCl(2)). Unlike HgCl(2), mercuric polysulfides exhibited two tightly binding sites to HSA and had little effect on viability of HK-2 cells.

Conclusion: Mercuric polysulfides, as the major dissolved components, may serve as the active species of cinnabar exhibiting pharmacological and/or toxicological effects. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30672653 ; 2001BA701A57
项目资助者: National Natural Science Foundation of China ; Tenth Five-Year Plan of Science and Technology of China
WOS记录号: WOS:000281325500027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54655
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Biol Chem, Beijing 100191, Peoples R China
2.Peking Univ, Sch Publ Hlth, Dept Toxicol, Beijing 100191, Peoples R China
3.Peking Univ, State Key Labs Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhou, Xinrui,Zeng, Kewu,Wang, Qi,et al. In vitro studies on dissolved substance of cinnabar: Chemical species and biological properties[J]. JOURNAL OF ETHNOPHARMACOLOGY,2010,131(1):196-202.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, Xinrui]'s Articles
[Zeng, Kewu]'s Articles
[Wang, Qi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, Xinrui]‘s Articles
[Zeng, Kewu]‘s Articles
[Wang, Qi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace