北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 药理学系  > 期刊论文
学科主题: 基础医学
题名:
Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro
作者: Sun, Li-Xin1,2; Chen, Li-Hua1,3; Lin, Zhi-Bin1; Qin, Ying1; Zhang, Jia-Qi1; Yang, Jing1; Ma, Jie1; Ye, Ting4; Li, Wei-Dong1
关键词: c-Myc ; Ganoderma lucidum polysaccharides ; IEC-6 cell line ; intestinal epithelium healing ; ODC
刊名: JOURNAL OF PHARMACY AND PHARMACOLOGY
发表日期: 2011-12-01
DOI: 10.1111/j.2042-7158.2011.01367.x
卷: 63, 期:12, 页:1595-1603
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: ORNITHINE-DECARBOXYLASE GENE ; C-MYC EXPRESSION ; MUCOSAL GROWTH ; RAS ONCOGENE ; CRYPT CELLS ; TGF-BETA ; MECHANISMS ; LYMPHOCYTES ; POLYAMINES ; RESTITUTION
英文摘要:

Objectives Restoration of epithelial continuity in the intestinal surface after extensive destruction is important since intestinal epithelial cells stand as a boundary between the body′s internal and external environment. Polysaccharides from Ganoderma lucidum (GlPS) may benefit intestinal epithelial wound healing in different aspects, which awaits clarification. To identify potential effects, a non-transformed small-intestinal epithelial cell line, IEC-6 cells, was used.

Methods Effects on epithelial cell proliferation, migration, morphology of differentiation and transforming growth factor beta (TGF-beta) protein expression, as well as the cellular ornithine decarboxylase (ODC) mRNA and c-Myc mRNA expression, were assessed, respectively, by MTT assay, wound model in vitro, observation under a microscope after hematoxylin and eosin staining, enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction assays.

Key findings It was shown that Gl-PS stimulated IEC-6 cell proliferation and migration significantly in a dose-dependent manner; 10 mu g/ml Gl-PS improved the morphology of differentiation in IEC-6 cells. Inefficacy in expression of TGF-beta in IEC-6 cells indicated a possible TGF-beta independent action of Gl-PS. However, Gl-PS increased ODC mRNA and c-Myc mRNA expression in a dose-dependent manner, indicating, at least partially possible involvement of ODC and c-Myc gene expression in improvement of intestinal wound healing.

Conclusions These results suggest the potential usefulness of Gl-PS to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.

语种: 英语
WOS记录号: WOS:000297418400012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54659
Appears in Collections:基础医学院_药理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Changsha Med Univ, Changsha, Hunan, Peoples R China
2.Tianjing Med Univ, Tianjin, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Basic Med Sch, Beijing 100191, Peoples R China
4.Chengde Med Coll, Affiliated Hosp, Chengde, Hebei Province, Peoples R China

Recommended Citation:
Sun, Li-Xin,Chen, Li-Hua,Lin, Zhi-Bin,et al. Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY,2011,63(12):1595-1603.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, Li-Xin]'s Articles
[Chen, Li-Hua]'s Articles
[Lin, Zhi-Bin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, Li-Xin]‘s Articles
[Chen, Li-Hua]‘s Articles
[Lin, Zhi-Bin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace