北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 药理学系  > 期刊论文
学科主题: 基础医学
题名:
Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro
作者: Sun, Li-Xin1,2; Chen, Li-Hua1,3; Lin, Zhi-Bin1; Qin, Ying1; Zhang, Jia-Qi1; Yang, Jing1; Ma, Jie1; Ye, Ting4; Li, Wei-Dong1
关键词: c-Myc ; Ganoderma lucidum polysaccharides ; IEC-6 cell line ; intestinal epithelium healing ; ODC
刊名: JOURNAL OF PHARMACY AND PHARMACOLOGY
发表日期: 2011-12-01
DOI: 10.1111/j.2042-7158.2011.01367.x
卷: 63, 期:12, 页:1595-1603
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: ORNITHINE-DECARBOXYLASE GENE ; C-MYC EXPRESSION ; MUCOSAL GROWTH ; RAS ONCOGENE ; CRYPT CELLS ; TGF-BETA ; MECHANISMS ; LYMPHOCYTES ; POLYAMINES ; RESTITUTION
英文摘要:

Objectives Restoration of epithelial continuity in the intestinal surface after extensive destruction is important since intestinal epithelial cells stand as a boundary between the body′s internal and external environment. Polysaccharides from Ganoderma lucidum (GlPS) may benefit intestinal epithelial wound healing in different aspects, which awaits clarification. To identify potential effects, a non-transformed small-intestinal epithelial cell line, IEC-6 cells, was used.

Methods Effects on epithelial cell proliferation, migration, morphology of differentiation and transforming growth factor beta (TGF-beta) protein expression, as well as the cellular ornithine decarboxylase (ODC) mRNA and c-Myc mRNA expression, were assessed, respectively, by MTT assay, wound model in vitro, observation under a microscope after hematoxylin and eosin staining, enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction assays.

Key findings It was shown that Gl-PS stimulated IEC-6 cell proliferation and migration significantly in a dose-dependent manner; 10 mu g/ml Gl-PS improved the morphology of differentiation in IEC-6 cells. Inefficacy in expression of TGF-beta in IEC-6 cells indicated a possible TGF-beta independent action of Gl-PS. However, Gl-PS increased ODC mRNA and c-Myc mRNA expression in a dose-dependent manner, indicating, at least partially possible involvement of ODC and c-Myc gene expression in improvement of intestinal wound healing.

Conclusions These results suggest the potential usefulness of Gl-PS to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.

语种: 英语
WOS记录号: WOS:000297418400012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54659
Appears in Collections:基础医学院_药理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Changsha Med Univ, Changsha, Hunan, Peoples R China
2.Tianjing Med Univ, Tianjin, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Basic Med Sch, Beijing 100191, Peoples R China
4.Chengde Med Coll, Affiliated Hosp, Chengde, Hebei Province, Peoples R China

Recommended Citation:
Sun, Li-Xin,Chen, Li-Hua,Lin, Zhi-Bin,et al. Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY,2011,63(12):1595-1603.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, Li-Xin]'s Articles
[Chen, Li-Hua]'s Articles
[Lin, Zhi-Bin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, Li-Xin]‘s Articles
[Chen, Li-Hua]‘s Articles
[Lin, Zhi-Bin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace