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学科主题: 临床医学
题名:
A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients
作者: Li, Min1; Tong, Xunliang2; Lv, Pu2; Feng, Baosheng1; Yang, Li1; Wu, Zheng1; Cui, Xinge1; Bai, Yu1; Huang, Yining2; Liu, Huwei1
关键词: Lipidomics ; Two-dimensional liquid chromatography-mass spectrometry ; Galactosylceramide ; Immunity ; Atherosclerosis
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-12-12
DOI: 10.1016/j.chroma.2014.10.094
卷: 1372, 页:110-119
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: LC-QTOF-MS ; T-CELLS ; PHOSPHOLIPIDS ; QUANTIFICATION ; ACTIVATION ; MECHANISMS ; DISEASE ; TISSUES ; SYSTEM ; IMMUNE
英文摘要:

A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4-8.0% and 0.010-0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5-2.8-fold increasing). It would be helpful to the further research of the atherosclerosis. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 21175005
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000347020900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54670
Appears in Collections:北京大学第一临床医学院_神经内科_期刊论文

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作者单位: 1.Peking Univ, Inst Analyt Chem, Coll Chem & Mol Engn,Minist Educ, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Neurol, Beijing 100034, Peoples R China

Recommended Citation:
Li, Min,Tong, Xunliang,Lv, Pu,et al. A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372:110-119.
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