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A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients
Li, Min1; Tong, Xunliang2; Lv, Pu2; Feng, Baosheng1; Yang, Li1; Wu, Zheng1; Cui, Xinge1; Bai, Yu1; Huang, Yining2; Liu, Huwei1
关键词Lipidomics Two-dimensional Liquid Chromatography-mass Spectrometry Galactosylceramide Immunity Atherosclerosis
刊名JOURNAL OF CHROMATOGRAPHY A
2014-12-12
DOI10.1016/j.chroma.2014.10.094
1372页:110-119
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]LC-QTOF-MS ; T-CELLS ; PHOSPHOLIPIDS ; QUANTIFICATION ; ACTIVATION ; MECHANISMS ; DISEASE ; TISSUES ; SYSTEM ; IMMUNE
英文摘要

A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4-8.0% and 0.010-0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5-2.8-fold increasing). It would be helpful to the further research of the atherosclerosis. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000347020900012
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54670
专题北京大学第一临床医学院_神经内科
作者单位1.Peking Univ, Inst Analyt Chem, Coll Chem & Mol Engn,Minist Educ, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Neurol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Li, Min,Tong, Xunliang,Lv, Pu,et al. A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372:110-119.
APA Li, Min.,Tong, Xunliang.,Lv, Pu.,Feng, Baosheng.,Yang, Li.,...&Liu, Huwei.(2014).A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients.JOURNAL OF CHROMATOGRAPHY A,1372,110-119.
MLA Li, Min,et al."A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients".JOURNAL OF CHROMATOGRAPHY A 1372(2014):110-119.
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