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学科主题: 基础医学
题名:
Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment
作者: Zhang, Yan; Li, Yin; Totsune, Kazuhito; Kikuchi, Kumi; Murakami, Osamu; Shibahara, Shigeki; Takahashi, Kazuhiro
刊名: PEPTIDES
发表日期: 2006-11-01
DOI: 10.1016/j.peptides.2006.04.014
卷: 27, 期:11, 页:3003-3006
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: COBALT-CHLORIDE ; ADRENOMEDULLIN ; RECEPTORS ; PROLIFERATION ; INDUCTION ; RELEASE ; CANCER ; LINES ; GENE
英文摘要:

Endothelin-1(ET-1) levels in the culture medium were considered to reflect the transcription of the ET-1 gene and the subsequent secretion of ET-1 from cultured cells. It has not been clarified how different ET-1 mRNA expression levels and immunoreactive (IR)-ET levels in the culture medium are in the cell culture system. We studied ET-1 mRNA expression levels and IR-ET levels in the medium of T98G glioblastoma cells treated with cytokines. T98G glioblastoma cells were cultured with cytokines (interferon-gamma 100 U/ml, tumor necrosis factor-alpha 20 ng/ml and interleukin-1 beta 10 ng/ml) under normoxia or hypoxia (1% O-2). Northern blot analysis showed that ET-1 mRNA expression levels were increased by tumor necrosis factor-a alone or a combination of tumor necrosis factor-alpha and interleukin-1 beta, or three cytokines, and the increase was further enhanced under hypoxia. Particularly, relative expression levels of ET-1 mRNA were significantly higher under hypoxia than in normoxia in the treatment with a combination of three cytokines. IR-ET levels in the medium were increased by treatment with tumor necrosis factor-alpha, interleukin-1 beta or a combination of tumor necrosis factor-alpha and interleukin-1 beta, or three cytokines. In contrast to the mRNA expression levels, IR-ET levels in the medium of T98G cells treated with a combination of three cytokines were rather decreased under hypoxia compared with those in normoxia. These findings indicate that hypoxia induces ET-1 mRNA expression in the treatment of three cytokines, but IR-ET levels in the medium do not reflect this induction in T98G glioblastoma cells. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000242318900057
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54677
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Tohoku Gakuin Univ, Sch Med, Dept Med, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Med, Dept Appl Physiol & Mol Biol, Sendai, Miyagi 9808575, Japan
3.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan
4.Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Sendai, Miyagi 9808578, Japan
5.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Yan,Li, Yin,Totsune, Kazuhito,et al. Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment[J]. PEPTIDES,2006,27(11):3003-3006.
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