北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 生理学与病理生理学系  > 期刊论文
学科主题: 基础医学
题名:
Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment
作者: Zhang, Yan; Li, Yin; Totsune, Kazuhito; Kikuchi, Kumi; Murakami, Osamu; Shibahara, Shigeki; Takahashi, Kazuhiro
刊名: PEPTIDES
发表日期: 2006-11-01
DOI: 10.1016/j.peptides.2006.04.014
卷: 27, 期:11, 页:3003-3006
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: COBALT-CHLORIDE ; ADRENOMEDULLIN ; RECEPTORS ; PROLIFERATION ; INDUCTION ; RELEASE ; CANCER ; LINES ; GENE
英文摘要:

Endothelin-1(ET-1) levels in the culture medium were considered to reflect the transcription of the ET-1 gene and the subsequent secretion of ET-1 from cultured cells. It has not been clarified how different ET-1 mRNA expression levels and immunoreactive (IR)-ET levels in the culture medium are in the cell culture system. We studied ET-1 mRNA expression levels and IR-ET levels in the medium of T98G glioblastoma cells treated with cytokines. T98G glioblastoma cells were cultured with cytokines (interferon-gamma 100 U/ml, tumor necrosis factor-alpha 20 ng/ml and interleukin-1 beta 10 ng/ml) under normoxia or hypoxia (1% O-2). Northern blot analysis showed that ET-1 mRNA expression levels were increased by tumor necrosis factor-a alone or a combination of tumor necrosis factor-alpha and interleukin-1 beta, or three cytokines, and the increase was further enhanced under hypoxia. Particularly, relative expression levels of ET-1 mRNA were significantly higher under hypoxia than in normoxia in the treatment with a combination of three cytokines. IR-ET levels in the medium were increased by treatment with tumor necrosis factor-alpha, interleukin-1 beta or a combination of tumor necrosis factor-alpha and interleukin-1 beta, or three cytokines. In contrast to the mRNA expression levels, IR-ET levels in the medium of T98G cells treated with a combination of three cytokines were rather decreased under hypoxia compared with those in normoxia. These findings indicate that hypoxia induces ET-1 mRNA expression in the treatment of three cytokines, but IR-ET levels in the medium do not reflect this induction in T98G glioblastoma cells. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000242318900057
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54677
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment.pdf(319KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Tohoku Gakuin Univ, Sch Med, Dept Med, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Med, Dept Appl Physiol & Mol Biol, Sendai, Miyagi 9808575, Japan
3.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan
4.Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Sendai, Miyagi 9808578, Japan
5.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Yan,Li, Yin,Totsune, Kazuhito,et al. Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment[J]. PEPTIDES,2006,27(11):3003-3006.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Yan]'s Articles
[Li, Yin]'s Articles
[Totsune, Kazuhito]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Yan]‘s Articles
[Li, Yin]‘s Articles
[Totsune, Kazuhito]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace