学科主题基础医学
Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment
Zhang, Yan; Li, Yin; Totsune, Kazuhito; Kikuchi, Kumi; Murakami, Osamu; Shibahara, Shigeki; Takahashi, Kazuhiro
刊名PEPTIDES
2006-11-01
DOI10.1016/j.peptides.2006.04.014
27期:11页:3003-3006
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]COBALT-CHLORIDE ; ADRENOMEDULLIN ; RECEPTORS ; PROLIFERATION ; INDUCTION ; RELEASE ; CANCER ; LINES ; GENE
英文摘要

Endothelin-1(ET-1) levels in the culture medium were considered to reflect the transcription of the ET-1 gene and the subsequent secretion of ET-1 from cultured cells. It has not been clarified how different ET-1 mRNA expression levels and immunoreactive (IR)-ET levels in the culture medium are in the cell culture system. We studied ET-1 mRNA expression levels and IR-ET levels in the medium of T98G glioblastoma cells treated with cytokines. T98G glioblastoma cells were cultured with cytokines (interferon-gamma 100 U/ml, tumor necrosis factor-alpha 20 ng/ml and interleukin-1 beta 10 ng/ml) under normoxia or hypoxia (1% O-2). Northern blot analysis showed that ET-1 mRNA expression levels were increased by tumor necrosis factor-a alone or a combination of tumor necrosis factor-alpha and interleukin-1 beta, or three cytokines, and the increase was further enhanced under hypoxia. Particularly, relative expression levels of ET-1 mRNA were significantly higher under hypoxia than in normoxia in the treatment with a combination of three cytokines. IR-ET levels in the medium were increased by treatment with tumor necrosis factor-alpha, interleukin-1 beta or a combination of tumor necrosis factor-alpha and interleukin-1 beta, or three cytokines. In contrast to the mRNA expression levels, IR-ET levels in the medium of T98G cells treated with a combination of three cytokines were rather decreased under hypoxia compared with those in normoxia. These findings indicate that hypoxia induces ET-1 mRNA expression in the treatment of three cytokines, but IR-ET levels in the medium do not reflect this induction in T98G glioblastoma cells. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000242318900057
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54677
专题北京大学基础医学院_生理学与病理生理学系
作者单位1.Tohoku Gakuin Univ, Sch Med, Dept Med, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Med, Dept Appl Physiol & Mol Biol, Sendai, Miyagi 9808575, Japan
3.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan
4.Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Sendai, Miyagi 9808578, Japan
5.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yan,Li, Yin,Totsune, Kazuhito,et al. Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment[J]. PEPTIDES,2006,27(11):3003-3006.
APA Zhang, Yan.,Li, Yin.,Totsune, Kazuhito.,Kikuchi, Kumi.,Murakami, Osamu.,...&Takahashi, Kazuhiro.(2006).Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment.PEPTIDES,27(11),3003-3006.
MLA Zhang, Yan,et al."Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment".PEPTIDES 27.11(2006):3003-3006.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Hypoxia increases en(319KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Yan]的文章
[Li, Yin]的文章
[Totsune, Kazuhito]的文章
百度学术
百度学术中相似的文章
[Zhang, Yan]的文章
[Li, Yin]的文章
[Totsune, Kazuhito]的文章
必应学术
必应学术中相似的文章
[Zhang, Yan]的文章
[Li, Yin]的文章
[Totsune, Kazuhito]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Hypoxia increases endothelin-1 mRNA expression but not immunoreactive endothelin in the medium of T98G glioblastoma cells under cytokine treatment.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。