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学科主题: 基础医学
题名:
The antifibrosis effect of adrenomedullin in human lung fibroblasts
作者: Hao, Shu-Ling1; Yu, Zhong-He1; Qi, Bao-Shen2; Luo, Ji-Zheng1; Wang, Wei-Ping3
关键词: adrenomedullin ; fibrosis ; hypoxia ; proliferation ; TGF-beta 1
刊名: EXPERIMENTAL LUNG RESEARCH
发表日期: 2011-12-01
DOI: 10.3109/01902148.2011.623823
卷: 37, 期:10, 页:615-626
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Respiratory System
研究领域[WOS]: Respiratory System
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; HUMAN DERMAL FIBROBLASTS ; PULMONARY-HYPERTENSION ; COLLAGEN-SYNTHESIS ; FACTOR-BETA ; HUMAN SKIN ; LIFE-SPAN ; HYPOXIA ; CELLS ; OXYGEN
英文摘要:

Adrenomedullin (AM) is a regulatory peptide involved in cellular proliferation and protein synthesis. The authors investigated AM and the AM receptor system in the human fetal lung fibroblasts (HFLFs), and assessed whether AM can inhibit proliferation and collagen synthesis in HFLFs under hypoxia. Fibroblasts were exposed to hypoxia (2% O-2) after the addition of AM. The effects of AM and transforming growth factor beta 1 (TGF-beta 1) on the proliferation of fibroblasts were determined by the methanethiosulfonate (MTS) assay. Total collagen synthesis was determined by [H-3] proline incorporation. TGF-beta 1 levels in the culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The concentration of intracellular calciumion ([Ca2+](i)) in fibroblasts was detected with a laser scanning confocal microscope. AM, adrenomedullin receptor (ADMR), calcitonin receptor-like receptor (CRLR), AM receptor chaperone receptor activity-modifying protein-1 (RAMP1), RAMP2, and RAMP3 were detected in the HFLFs. The hypoxia-induced increases in cell proliferation, collagen synthesis, and TGF-beta 1 production were inhibited by AM. AM also inhibited proliferation and collagen synthesis in fibroblasts induced by TGF-beta 1. AM caused a decrease of the hypoxia-induced [Ca2+](i) in fibroblasts. This study suggests that AM is produced by HFLFs and AM may function as an antifibrosis factor that protects cells from hypoxic pulmonary damage through its receptors.

语种: 英语
所属项目编号: 30070336
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000297057600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54717
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Beijing PLA, Mil Gen Hosp, Dept Resp Dis, Beijing, Peoples R China
2.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Physiol & Pathophysiol, Peking Union Med Coll, Beijing 100730, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China

Recommended Citation:
Hao, Shu-Ling,Yu, Zhong-He,Qi, Bao-Shen,et al. The antifibrosis effect of adrenomedullin in human lung fibroblasts[J]. EXPERIMENTAL LUNG RESEARCH,2011,37(10):615-626.
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