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学科主题: 基础医学
题名:
Murine Monoclonal Antibodies Generated Against Mouse/Rat Hemokinin-1
作者: Jin, Liang1; Jin, Bo-quan1; Song, Chao-jun1; Zhang, Yu2
刊名: HYBRIDOMA
发表日期: 2009-08-01
DOI: 10.1089/hyb.2009.0009
卷: 28, 期:4, 页:259-267
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: MAMMALIAN TACHYKININ PEPTIDE ; P-LIKE FUNCTION ; SUBSTANCE-P ; RAT/MOUSE HEMOKININ-1 ; IDENTIFICATION ; LYMPHOPOIESIS ; LOCALIZATION ; MECHANISMS ; RECEPTOR ; CELLS
英文摘要:

The mouse/rat hemokinin-1 (m/rHK-1) was discovered nearly 9 years ago. This molecule is a peptide comprising 11 amino acids. The m/rHK-1 was found to be mainly expressed in central immune organs like bone marrow, and was proven to have lymphopoietic roles in B and T lymphocyte development. m/rHK-1 was also reported to have analgesic roles in rat spinal cord, in addition to other functions such as relaxing activity on coronary artery. Unlike its analogues SP, NKA, and NKB, m/rHK-1 does not express in the nervous system. To further study the distribution and function of m/rHK-1, we carried out conventional immunization and cell fusion procedures to acquire the hybridomas secreting specific monoclonal antibodies to m/rHK-1. In the 17 positive clones obtained, three antibodies named 1B12, 2B4, and 4G5 were shown representative in cross-reactivity against m/rHK-1 and its analogues by indirect ELISA, competitive indirect ELISA, and immunofluorescence assays. Among the three clones, the 2B4 monoclonal antibody appeared to be the high-titered and specific clone to m/rHK-1. Monoclonal antibodies to m/rHK-1 will function as good tools in the physiological study of m/rHK-1 in the near future.

语种: 英语
WOS记录号: WOS:000268827000005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54729
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Fourth Mil Med Univ, Dept Immunol, Xian 710032, Peoples R China
2.Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Jin, Liang,Jin, Bo-quan,Song, Chao-jun,et al. Murine Monoclonal Antibodies Generated Against Mouse/Rat Hemokinin-1[J]. HYBRIDOMA,2009,28(4):259-267.
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