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Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides
Tang, XinJing1,2; Su, Meng1; Yu, LiLi3; Lv, Cong1; Wang, Jie2; Li, ZhongJin3
刊名NUCLEIC ACIDS RESEARCH
2010-06-01
DOI10.1093/nar/gkq079
38期:11页:3848-3855
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]REGULATING GENE-EXPRESSION ; HUMAN LEUKEMIA-CELLS ; ZEBRAFISH EMBRYOS ; REMOTE-CONTROL ; CAGED SIRNAS ; LIGHT ; DNA ; INTERFERENCE ; OLIGONUCLEOTIDES ; HYBRIDIZATION
英文摘要

Caged antisense oligodeoxynucleotides (asODNs) are synthesized by linking two ends of linear oligodeoxynucleotides using a photocleavable linker. Two of them (H30 and H40) have hairpin-like structures which show a large difference in thermal stability (delta T(m) = 17.5 degrees C and 11.6 degrees C) comparing to uncaged ones. The other three (C20, C30 and C40) without stable secondary structures have the middle 20 deoxynucleotides complementary to 40-mer RNA. All caged asODNs have restricted opening which provides control over RNA/asODN interaction. RNase H assay results showed that 40-mer RNA digestion could be photo-modulated 2- to 3-fold upon light-activation with H30, H40, C30 and C40, while with C20, RNA digestion was almost not detectable; however, photo-activation triggered > 20-fold increase of RNA digestion. And gel shift assays showed that it needed > 0.04 mu M H40 and 0.5 mu M H30 to completely bind 0.02 mu M 40-mer RNA, and for C40 and C30, it needed > 0.2 mu M and 0.5 mu M for 0.02 mu M 40-mer RNA binding. However, even 4 mu M C20 was not able to fully bind the same concentration of 40-mer RNA. By simple adjustment of ring size of caged asODNs, we could successfully photoregulate their hybridization with mRNA and target RNA hydrolysis by RNase H with light activation.

语种英语
WOS记录号WOS:000279188800039
项目编号bmu-2009137-121
资助机构State Key Laboratory of Natural and Biomimetic Drugs ; Peking University "985&prime ; &prime ; foundation
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54744
专题北京大学药学院
北京大学医学部管理机构_医学部
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Shaanxi Univ Sci & Technol, Coll Chem & Chem Engn, Xian 710021, Shaanxi, Peoples R China
推荐引用方式
GB/T 7714
Tang, XinJing,Su, Meng,Yu, LiLi,et al. Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides[J]. NUCLEIC ACIDS RESEARCH,2010,38(11):3848-3855.
APA Tang, XinJing,Su, Meng,Yu, LiLi,Lv, Cong,Wang, Jie,&Li, ZhongJin.(2010).Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides.NUCLEIC ACIDS RESEARCH,38(11),3848-3855.
MLA Tang, XinJing,et al."Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides".NUCLEIC ACIDS RESEARCH 38.11(2010):3848-3855.
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