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学科主题: 基础医学
题名:
Hemokinin-1 As an Adjuvant Molecule Enhancing Humoral and Memory Responses to HBsAg DNA Vaccination
作者: Chen, Xuan1; Zhang, Wang2,3; Gao, Wenqing1; Zou, Qiang1; Feng, Congcong1; Liu, Hu1; Zhou, Chenliang1; Zhang, Yu2,3; Wang, Bin1,4
刊名: VIRAL IMMUNOLOGY
发表日期: 2012-08-01
DOI: 10.1089/vim.2012.0015
卷: 25, 期:4, 页:289-296
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Virology
研究领域[WOS]: Immunology ; Virology
关键词[WOS]: HEPATITIS-B-VIRUS ; CELL PROLIFERATION ; IMMUNE-RESPONSES ; IMMUNIZATION ; TACHYKININ ; VACCINES ; VIVO
英文摘要:

Incorporation of molecular adjuvants into DNA vaccines is often used to improve the induction of immune responses, but few approaches aim to specifically activate B cells for an enhanced humoral response only. Hemokinin-1 (HK-1) is a factor that activates B cells for proliferation, survival, differentiation into plasma cells, and Ab production. Therefore, we investigated if it may be used as a molecular adjuvant for DNA vaccines to elicit strong humoral and memory responses. The HK-1 coding sequence was sub-cloned as single or triple copies in-frame downstream of S2 HBsAg in the proVAX/S2 construct. Compared to mice immunized with proVAX/S2 or proVAX/S2-HK-1, proVAX/S2-3HK-1 induced a higher level of IgG production, a higher percentage of differentiated antibody-secreting plasma cells, and a higher level of T-cell proliferation. Furthermore, a higher proportion of B cells had the B220(+) CD27(+) phenotype in these groups, and specific antigen re-challenge induced a higher level of total IgG production 60 d after the last immunization, suggesting that the use of HK-1 as an adjuvant promoted immunological memory. Taken together, these results suggest that using HK-1 as an adjuvant molecule could enhance the immunogenicity of HBsAg DNA vaccines, and result in stronger humoral and memory responses. Therefore, HK-1 may lead to the development of a novel humoral-biased molecular adjuvant for an HBsAg DNA vaccine against hepatitis B infection.

语种: 英语
所属项目编号: 30771602
项目资助者: National High-Tech 863 Project of China ; National Nature Science Foundation
WOS记录号: WOS:000307729400008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54757
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Hlth, Key Lab Med Immunol, Beijing 100871, Peoples R China
4.Fudan Univ, Shanghai Med Coll, Minist Hlth, Key Lab Med Mol Virol, Shanghai 200433, Peoples R China

Recommended Citation:
Chen, Xuan,Zhang, Wang,Gao, Wenqing,et al. Hemokinin-1 As an Adjuvant Molecule Enhancing Humoral and Memory Responses to HBsAg DNA Vaccination[J]. VIRAL IMMUNOLOGY,2012,25(4):289-296.
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