IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture
Xu, Guangwei1,2,3; Gao, Zhenchao3,4; He, Wenhui3; Ma, Yanting3; Feng, Xiaofeng3; Cai, Tao3; Lu, Fengmin5; Liu, Li1,2; Li, Wenhui3
关键词Hepatitis B virus MicroRNA-122 Tupaia Hepatocyte RNA deep sequencing MicroRNA database Sodium taurocholate cotransporting polypeptide (NTCP) De novo infection Pathogenesis
刊名VIROLOGY
2014-01-05
DOI10.1016/j.viro1.2013.10.016
448期:2页:247-254
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
资助者Chinese Ministry of Science and Technology ; Beijing Municipal Government ; Chinese Ministry of Science and Technology ; Beijing Municipal Government
研究领域[WOS]Virology
关键词[WOS]LIVER-SPECIFIC MICRORNA ; TUPAIA HEPATOCYTES ; REPLICATION ; IDENTIFICATION ; SUPPRESSION ; DNA
英文摘要

Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2-NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest that de novo HBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
所属项目编号2010CB530101 ; 2011CB812501
资助者Chinese Ministry of Science and Technology ; Beijing Municipal Government ; Chinese Ministry of Science and Technology ; Beijing Municipal Government
WOS记录号WOS:000328710800026
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54786
专题北京大学基础医学院_病原生物学系
作者单位1.Peking Union Med Coll, Sch Basic Med Sci, Beijing 100005, Peoples R China
2.Chinese Acad Med Sci, Beijing 100005, Peoples R China
3.Natl Inst Biol Sci, Beijing 102206, Peoples R China
4.Peking Univ, Sch Life Sci, Grad Program, Beijing 100871, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
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Xu, Guangwei,Gao, Zhenchao,He, Wenhui,et al. microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture[J]. VIROLOGY,2014,448(2):247-254.
APA Xu, Guangwei.,Gao, Zhenchao.,He, Wenhui.,Ma, Yanting.,Feng, Xiaofeng.,...&Li, Wenhui.(2014).microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture.VIROLOGY,448(2),247-254.
MLA Xu, Guangwei,et al."microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture".VIROLOGY 448.2(2014):247-254.
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