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学科主题: 基础医学
题名:
microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture
作者: Xu, Guangwei1,2,3; Gao, Zhenchao3,4; He, Wenhui3; Ma, Yanting3; Feng, Xiaofeng3; Cai, Tao3; Lu, Fengmin5; Liu, Li1,2; Li, Wenhui3
关键词: Hepatitis B virus ; MicroRNA-122 ; Tupaia ; Hepatocyte ; RNA deep sequencing ; MicroRNA database ; Sodium taurocholate cotransporting polypeptide (NTCP) ; De novo infection ; Pathogenesis
刊名: VIROLOGY
发表日期: 2014-01-05
DOI: 10.1016/j.viro1.2013.10.016
卷: 448, 期:2, 页:247-254
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: LIVER-SPECIFIC MICRORNA ; TUPAIA HEPATOCYTES ; REPLICATION ; IDENTIFICATION ; SUPPRESSION ; DNA
英文摘要:

Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2-NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest that de novo HBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 2010CB530101 ; 2011CB812501
项目资助者: Chinese Ministry of Science and Technology ; Beijing Municipal Government
WOS记录号: WOS:000328710800026
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54786
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Union Med Coll, Sch Basic Med Sci, Beijing 100005, Peoples R China
2.Chinese Acad Med Sci, Beijing 100005, Peoples R China
3.Natl Inst Biol Sci, Beijing 102206, Peoples R China
4.Peking Univ, Sch Life Sci, Grad Program, Beijing 100871, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China

Recommended Citation:
Xu, Guangwei,Gao, Zhenchao,He, Wenhui,et al. microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture[J]. VIROLOGY,2014,448(2):247-254.
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