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学科主题: 临床医学
题名:
A peptide fusion protein inhibits angiogenesis and tumor growth by blocking VEGF binding to KDR
作者: Lei, HT; Shou, CC; Liu, XY; He, LW; Wu, J; Jiang, BH; Liu, MS; Yang, JS
关键词: vascular endothelial cell growth factor (VEGF) ; VEGFR ; peptide ; antiangiogenesis
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2002-04-01
卷: 47, 期:8, 页:682-687
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CANCER
英文摘要:

Vascular endothelial growth factor (VEGF) binding to its tyrosine kinase receptors (KDR/FLKI, Flt-1) induces angiogenesis. In search of the peptides blocking VEGF binding to its receptor KDR/FLK1 to inhibit tumor-angiogenesis and growth, we screened a phage display peptide library with KDR as target protein, and some candidate peptides were isolated. In this study, we cloned the DNA fragment coding the peptide K237 from the library, into a vector pQE42 to express fusion protein DHFR-K237 in E. coli M15. The affection of fusion protein DHFR-K237 on endothelial cell proliferation and angiogenesis was investigated. In vitro, DHFR-K237 could completely block VEGF binding to KDR and significantly inhibit the VEGF-mediated proliferation of the human vascular endothelial cells. In vivo, DHFR-K237 inhibited angiogenesis in chick embryo chorioa-llantoric membrane and tumor growth in nude mice. These results suggest that K237 is an effective antagonist of VEGF binding to KDR, and could be a potential agent for cancer biotherapy.

语种: 英语
WOS记录号: WOS:000174980800014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54793
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
2.Chinese Acad Med Sci, Inst Med Plant, Peking Union Med Coll, Beijing 100094, Peoples R China

Recommended Citation:
Lei, HT,Shou, CC,Liu, XY,et al. A peptide fusion protein inhibits angiogenesis and tumor growth by blocking VEGF binding to KDR[J]. CHINESE SCIENCE BULLETIN,2002,47(8):682-687.
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