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学科主题: 临床医学
题名:
Apoptotic-like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus Rhizopus arrhizus
作者: Wang, Sibu; Li, Ruoyu; Yu, Jin
关键词: Rhizopus arrhizus ; Hydrogen peroxide ; Amphotericin B ; Apoptosis
刊名: MYCOSES
发表日期: 2014-12-01
DOI: 10.1111/myc.12240
卷: suppl.3, 期:SI, 页:25-30
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology ; Mycology
研究领域[WOS]: Dermatology ; Mycology
关键词[WOS]: PROGRAMMED CELL-DEATH ; FARNESOL-INDUCED APOPTOSIS ; CANDIDA-ALBICANS ; ASPERGILLUS-NIDULANS ; STATIONARY-PHASE ; YEAST ; MUCORMYCOSIS ; ZYGOMYCOSIS ; INDUCTION ; DISEASE
英文摘要:

Rhizopus is the most common genus of invasive mucormycosis, whose prognosis and outcome was not improved over the past decades. We studied the apoptotic-like phenotype in Rhizopus arrhizus exposed to hydrogen peroxide (H2O2) and amphotericin B (AMB). The strain provided by Fungal Genetic Stock centre was studied about the apoptotic-like phenotype treated with different concentrations of H2O2 and AMB, and then analyzed by fluorescent microscopy (observed by Annexin-V/FITC and TUNEL staining), flow cytometry (stained with DHR123/PI), and DNA agarose gel electrophores. When R. arrhizus was treated with H2O2 and AMB, there was a loss of viability associated with different phenotype of apoptosis makers. Membrane externalization of phosphatidylserine (PS) on the cell surface, DNA fragmentation, chromatin condensation can be induced and observed obviously by Annexin-V/FITC, DAPI and TUNEL staining. DNA smear not DNA ladder was also visible in R. arrhizus. Flowcytometry of R. arrhizus cells revealed not only the increase of apoptosis cell stained with DHR123 under the nonfungicida doses but dead cells stained with PI under the fungicida concentrations.This study indicated that both H2O2 and AMB could induce the apoptotic-like phenotype in R. arrhizus.

语种: 英语
所属项目编号: 81371783
项目资助者: National Natural Science Foundation from the National Natural Science Foundation of China
WOS记录号: WOS:000345993900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54799
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Sibu,Li, Ruoyu,Yu, Jin. Apoptotic-like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus Rhizopus arrhizus[J]. MYCOSES,2014,suppl.3(SI):25-30.
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