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学科主题临床医学
Apoptotic-like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus Rhizopus arrhizus
Wang, Sibu; Li, Ruoyu; Yu, Jin
关键词Rhizopus Arrhizus Hydrogen Peroxide Amphotericin b Apoptosis
刊名MYCOSES
2014-12-01
DOI10.1111/myc.12240
suppl.3期:SI页:25-30
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology ; Mycology
资助者National Natural Science Foundation from the National Natural Science Foundation of China ; National Natural Science Foundation from the National Natural Science Foundation of China
研究领域[WOS]Dermatology ; Mycology
关键词[WOS]PROGRAMMED CELL-DEATH ; FARNESOL-INDUCED APOPTOSIS ; CANDIDA-ALBICANS ; ASPERGILLUS-NIDULANS ; STATIONARY-PHASE ; YEAST ; MUCORMYCOSIS ; ZYGOMYCOSIS ; INDUCTION ; DISEASE
英文摘要

Rhizopus is the most common genus of invasive mucormycosis, whose prognosis and outcome was not improved over the past decades. We studied the apoptotic-like phenotype in Rhizopus arrhizus exposed to hydrogen peroxide (H2O2) and amphotericin B (AMB). The strain provided by Fungal Genetic Stock centre was studied about the apoptotic-like phenotype treated with different concentrations of H2O2 and AMB, and then analyzed by fluorescent microscopy (observed by Annexin-V/FITC and TUNEL staining), flow cytometry (stained with DHR123/PI), and DNA agarose gel electrophores. When R. arrhizus was treated with H2O2 and AMB, there was a loss of viability associated with different phenotype of apoptosis makers. Membrane externalization of phosphatidylserine (PS) on the cell surface, DNA fragmentation, chromatin condensation can be induced and observed obviously by Annexin-V/FITC, DAPI and TUNEL staining. DNA smear not DNA ladder was also visible in R. arrhizus. Flowcytometry of R. arrhizus cells revealed not only the increase of apoptosis cell stained with DHR123 under the nonfungicida doses but dead cells stained with PI under the fungicida concentrations.This study indicated that both H2O2 and AMB could induce the apoptotic-like phenotype in R. arrhizus.

语种英语
所属项目编号81371783
资助者National Natural Science Foundation from the National Natural Science Foundation of China ; National Natural Science Foundation from the National Natural Science Foundation of China
WOS记录号WOS:000345993900006
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54799
Collection北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Wang, Sibu,Li, Ruoyu,Yu, Jin. Apoptotic-like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus Rhizopus arrhizus[J]. MYCOSES,2014,suppl.3(SI):25-30.
APA Wang, Sibu,Li, Ruoyu,&Yu, Jin.(2014).Apoptotic-like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus Rhizopus arrhizus.MYCOSES,suppl.3(SI),25-30.
MLA Wang, Sibu,et al."Apoptotic-like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus Rhizopus arrhizus".MYCOSES suppl.3.SI(2014):25-30.
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