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学科主题: 药学
题名:
Nucleolin-targeting liposomes guided by aptamer AS1411 for the delivery of siRNA for the treatment of malignant melanomas
作者: Li, Liyu; Hou, Jianjun; Liu, Xinjie; Guo, Yujia; Wu, Yun; Zhang, Lihe; Yang, Zhenjun
关键词: Nucleolin As1411 ; Liposomes siRNA delivery ; Melanomas ; Therapeutic effect
刊名: BIOMATERIALS
发表日期: 2014-04-01
DOI: 10.1016/j.biomaterials.2014.01.019
卷: 35, 期:12, 页:3840-3850
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: PLGA-PEG NANOPARTICLES ; PROSTATE-CANCER CELLS ; DRUG-DELIVERY ; ANTIPROLIFERATIVE ACTIVITY ; THERAPY ; SYSTEM ; GROWTH ; OLIGONUCLEOTIDES ; TRANSFECTION ; CYTOTOXICITY
英文摘要:

BRAF gene mutation is found in more than 60% of malignant melanomas, which are difficult to treat. In this study, a new tumor-targeting liposome was developed to deliver anti-BRAF siRNA (siBraf) for the treatment of melanomas. Nucleolin is overexpressed on the surface of cancer cells. AS1411, an aptamer showing specific binding to nucleolin, was conjugated to PEGylated cationic liposome as the targeting probe ASLP (AS1411 PEG-liposome). The ASLP/siRNA complex was formed through electrostatic interaction between ASLP and siRNA. The binding of A51411 to the surface of PEGylated liposomes was confirmed by gel electrophoresis and capillary electrophoresis. Real-time PCR and Western blot analysis showed that ASLP/siBraf exhibited strong silencing activity of BRAE gene. The much higher accumulation of the siRNA in tumor cells comparing with normal cells indicated that ASLP displayed excellent tumortargeting capability. Notably, ASLP/siBraf showed significant silencing activity in A375 tumor xenograft mice and inhibited the melanoma growth. These results suggested that the new nucleolin-targeted siRNA delivery system by AS1411 may have the potential for the treatment of melanoma. (c) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2012CB720604 ; 2012AA022501 ; 20092001 ; 2009ZX09310-001
项目资助者: National Basic Research Program of China ; NSFC projects ; New Drug RD
WOS记录号: WOS:000332431700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54806
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Li, Liyu,Hou, Jianjun,Liu, Xinjie,et al. Nucleolin-targeting liposomes guided by aptamer AS1411 for the delivery of siRNA for the treatment of malignant melanomas[J]. BIOMATERIALS,2014,35(12):3840-3850.
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