|The target antigens of antineutrophil cytoplasmic antibodies (ANCA) induced by propylthiouracil|
|Gao, Ying; Chen, Min; Ye, Hua; Guo, Xiao-Hui; Zhao, Ming-hui; Wang, Hai-yan|
|关键词||Antineutrophil Cytoplasmic Antibodies Vasculitis Propylthiouracil Hyperthyroidism Target Antigen|
|WOS标题词||Science & Technology|
|类目[WOS]||Immunology ; Pharmacology & Pharmacy|
|研究领域[WOS]||Immunology ; Pharmacology & Pharmacy|
|关键词[WOS]||OF-THE-LITERATURE ; ANTITHYROID DRUGS ; AUTOANTIBODIES ANCA ; SYSTEMIC VASCULITIS ; POSITIVE VASCULITIS ; INCREASING PROTEIN ; CHINESE PATIENTS ; GRAVES-DISEASE ; MYELOPEROXIDASE ; HYPERTHYROIDISM|
Objective: Antineutrophil cytoplasmic antibody (ANCA) has been well documented in association with propylthiouracil (PTU), and some patients with PTU-induced ANCA also develop clinical vasculitis. The aim of the current study was to detect ANCA specificities in sera from patients with PTU-induced ANCA with and without clinical vasculitis.
Methods: Sera from 65 patients with PTU-induced ANCA were collected, and 27 of these patients were diagnosed with PTU-induced ANCA associated systemic vasculitis (AASV). Indirect immunofluorescence assay and antigen-specific ELISAs were used to detect ANCA and their antigen specificities. The seven known target antigens included myeloperoxidase (MPO), protemase 3, human leukocyte elastase, lactoferrin, cathepsin G, azurocidin and bactericidal/pen-neability-increasing protein (BPI).
Results: In IIF assay, P-ANCA was found in 58/65 (89.2%) sera, C-ANCA in two, both P-ANCA and C-ANCA in five, respectively. MPO (60%) and lactoferrin (63.1%) were the two most common target antigens detected in sera from all the patients. 25/27 sera from patients with PTU-induced AASV recognized multiple target antigens, which was significantly higher than those (13/38) from patients without (P < 0.001). Except anti-BPI antibodies, the prevalence of antibodies against the other six target antigens was significantly higher in patients with clinical vasculitis than that in patients without (P < 0.05, respectively).
Conclusion: Antibodies against multiple ANCA specific antigens, especially the antigens rather than MPO and PR3, might be the characteristic of PTU-induced ANCA. Patients with antibodies against more ANCA specific antigens might be at increased risk of developing overt clinical vasculitis. The mechanism of ANCA production in PTU-induced cases was different from that in primary AASV (c) 2006 Elsevier B.V. All rights reserved.
|作者单位||1.Peking Univ, First Hosp, Dept Nephrol, Beijing 100034, Peoples R China|
2.Peking Univ, First Hosp, Dept Endocrinol, Beijing 100034, Peoples R China
|Gao, Ying,Chen, Min,Ye, Hua,et al. The target antigens of antineutrophil cytoplasmic antibodies (ANCA) induced by propylthiouracil[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2007,7(1):55-60.|
|APA||Gao, Ying,Chen, Min,Ye, Hua,Guo, Xiao-Hui,Zhao, Ming-hui,&Wang, Hai-yan.(2007).The target antigens of antineutrophil cytoplasmic antibodies (ANCA) induced by propylthiouracil.INTERNATIONAL IMMUNOPHARMACOLOGY,7(1),55-60.|
|MLA||Gao, Ying,et al."The target antigens of antineutrophil cytoplasmic antibodies (ANCA) induced by propylthiouracil".INTERNATIONAL IMMUNOPHARMACOLOGY 7.1(2007):55-60.|