IR@PKUHSC  > 北京大学口腔医学院  > 综合治疗科
学科主题口腔医学
Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro
Pan, Jie1; Sun, Ke3; Liang, Yongdong; Sun, Peng; Yang, Xiaohui3; Wang, Jing3; Zhang, Jue2; Zhu, Weidong4; Fang, Jing; Becker, Kurt H.5
关键词Biofilm Cold Plasma Disinfection Enterococcus Faecalis Root Canal
刊名JOURNAL OF ENDODONTICS
2013
DOI10.1016/j.joen.2012.08.017
39期:1页:105-110
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者College of Engineering, Peking University ; Medicine School of Peking University ; Bioelectrics Inc (Raleigh, NC) ; MST Program of International Science and Technology Cooperation ; National Basic Research Program ; Electro-Energetic Physics Program of the United States Air Force Office of Scientific Research ; College of Engineering, Peking University ; Medicine School of Peking University ; Bioelectrics Inc (Raleigh, NC) ; MST Program of International Science and Technology Cooperation ; National Basic Research Program ; Electro-Energetic Physics Program of the United States Air Force Office of Scientific Research
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]ATMOSPHERIC-PRESSURE AIR ; CALCIUM HYDROXIDE ; ANTIMICROBIAL ACTIVITY ; BACTERIAL POPULATION ; SODIUM-HYPOCHLORITE ; DENTINAL TUBULES ; INSTRUMENTATION ; TEETH ; IRRIGATION ; STERILIZATION
英文摘要

Introduction: Complete sterilization of an infected root canal is an important challenge in endodontic treatment. Traditional methods often cannot achieve high-efficiency sterilization because of the complexity of the root canal system. The objective of the study was to investigate in vitro the feasibility of using a cold plasma treatment of a root canal infected with Enterococcus faecalis biofilms. Methods: Seventy single-root teeth infected with E. faecalis biofilms were divided into 7 groups. Group 1 served as the negative control group (no treatment), and group 7 was the positive control group with teeth treated with calcium hydroxide intracanal medication for 7 days. Groups 2 to 6 included teeth treated by cold plasma for 2, 4, 6, 8, and 10 minutes, respectively. The disinfection of the E. faecalis biofilm was evaluated by colony-forming unit (CFU) counting. Scanning electron microscopy was used to evaluate the structural changes of the E. faecalis biofilm before and after plasma treatment. Confocal scanning laser microscopy was used to investigate the vitality of the microorganisms in the biofilm before and-after plasma treatment. Results: A significant decrease in the number of CFUs was observed after prolonged cold plasma treatment (based on the statistical analysis of the teeth in groups 2-6). Compared with the positive control group, cold plasma treatment of 8 or 10 minutes (groups 5 and 6) had a significantly higher antimicrobial efficacy (P < .05). The scanning electron microscopic analysis showed that the bacteria membrane was ruptured, and the structure of the biofilm was fully destroyed by the plasma. Confocal scanning laser microscopic studies indicated that the plasma treatment induced E. faecalis death and destruction of the biofilm. Conclusions: The cold plasma-had a high efficiency in disinfecting the E. faecalis biofilms in in vitro dental root Canal treatment. (J Endod 2013;39:105-110)

语种英语
所属项目编号2009DFB30370 ; 2007CB935602 ; FA9550-08-1-0332
资助者College of Engineering, Peking University ; Medicine School of Peking University ; Bioelectrics Inc (Raleigh, NC) ; MST Program of International Science and Technology Cooperation ; National Basic Research Program ; Electro-Energetic Physics Program of the United States Air Force Office of Scientific Research ; College of Engineering, Peking University ; Medicine School of Peking University ; Bioelectrics Inc (Raleigh, NC) ; MST Program of International Science and Technology Cooperation ; National Basic Research Program ; Electro-Energetic Physics Program of the United States Air Force Office of Scientific Research
WOS记录号WOS:000313378900020
引用统计
被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54847
专题北京大学口腔医学院_综合治疗科
作者单位1.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China
2.St Peters Coll, Dept Appl Sci & Technol, Jersey City, NJ USA
3.NYU, Polytech Inst, Dept Appl Phys, Brooklyn, NY USA
4.Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent, Beijing 100871, Peoples R China
5.Peking Univ, Coll Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Pan, Jie,Sun, Ke,Liang, Yongdong,et al. Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro[J]. JOURNAL OF ENDODONTICS,2013,39(1):105-110.
APA Pan, Jie.,Sun, Ke.,Liang, Yongdong.,Sun, Peng.,Yang, Xiaohui.,...&Becker, Kurt H..(2013).Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro.JOURNAL OF ENDODONTICS,39(1),105-110.
MLA Pan, Jie,et al."Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro".JOURNAL OF ENDODONTICS 39.1(2013):105-110.
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