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学科主题: 基础医学
题名:
Genomic diversification among archival strains of Salmonella enterica serovar Typhimurium LT7
作者: Liu, GR; Edwards, K; Eisenstark, A; Fu, YM; Liu, WQ; Sanderson, KE; Johnston, RN; Liu, SL
刊名: JOURNAL OF BACTERIOLOGY
发表日期: 2003-04-01
DOI: 10.1128/JB.185.7.2131-2142.2003
卷: 185, 期:7, 页:2131-2142
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: DETERMINING DIVERGENCE TIMES ; CLEAVAGE MAP ; ESCHERICHIA-COLI ; RIBOSOMAL-RNA ; MISMATCH REPAIR ; CHLAMYDOMONAS-EUGAMETOS ; PROTEIN CLOCK ; GENE ; ENDONUCLEASE ; SEQUENCE
英文摘要:

To document genomic changes during long periods of storage, we analyzed Salmonella enterica serovar Typhimurium LT7, a mutator strain that was previously reported to have higher rates of mutations compared to other serovar Typhimurium strains such as LT2. Upon plating directly from sealed agar stabs that had been stocked at room temperature for up to four decades, many auxotrophic mutants derived from LT7 gave rise to colonies of different sizes. Restreaking from single colonies consistently yielded colonies of diverse sizes even when we repeated single-colony isolation nine times. Colonies from the first plating had diverse genomic changes among and even within individual vials, including translocations, inversions, duplications, and point mutations, which were detected by rare-cutting endonuclease analysis with pulsed-field gel electrophoresis. Interestingly, even though the colony size kept diversifying, all descendents of the same single colonies from the first plating had the same sets of detected genomic changes. We did not detect any colony size or genome structure diversification in serovar Typhimurium LT7 stocked at -70degreesC or in serovar Typhimurium LT2 stocked either at -70degreesC or at room temperature. These results suggest that, although colony size diversification occurred during rapid growth, all detected genomic changes took place during the storage at room temperature and were carried over to their descendents without further changes during rapid growth in rich medium. We constructed a genomic cleavage map on the LT7 strain that had been stocked at -70degreesC and located all of the detected genomic changes on the map. We speculated on the significance of mutators for survival and evolution under environmentally stressed conditions.

语种: 英语
WOS记录号: WOS:000181703700008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54849
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada
2.Univ Calgary, Dept Sci Biol, Calgary, AB T2N 4N1, Canada
3.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB T2N 4N1, Canada
4.Ctr Canc Res, Columbia, MO 65201 USA
5.Univ Missouri, Columbia, MO 65201 USA
6.Harbin Med Univ, Dept Microbiol, Harbin 150086, Peoples R China
7.Peking Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100083, Peoples R China

Recommended Citation:
Liu, GR,Edwards, K,Eisenstark, A,et al. Genomic diversification among archival strains of Salmonella enterica serovar Typhimurium LT7[J]. JOURNAL OF BACTERIOLOGY,2003,185(7):2131-2142.
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