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学科主题: 基础医学
题名:
Lack of Protein 4.1G Causes Altered Expression and Localization of the Cell Adhesion Molecule Nectin-Like 4 in Testis and Can Cause Male Infertility
作者: Yang, Shaomin2,4; Weng, Haibo2; Chen, Lixiang2; Guo, Xinhua2; Parra, Marilyn2; Conboy, John5; Debnath, Gargi2; Lambert, Amy J.3; Peters, Luanne L.3; Baines, Anthony J.6; Mohandas, Narla2; An, Xiuli1,2
刊名: MOLECULAR AND CELLULAR BIOLOGY
发表日期: 2011-06-01
DOI: 10.1128/MCB.01105-10
卷: 31, 期:11, 页:2276-2286
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: QUANTITATIVE PCR DATA ; TIME RT-PCR ; JUNCTION DYNAMICS ; FUNCTIONAL-CHARACTERIZATION ; IMMUNOGLOBULIN SUPERFAMILY ; SURFACE LOCALIZATION ; MEMBRANE SKELETON ; GENETIC-VARIATION ; 129 SUBSTRAINS ; SERTOLI-CELLS
英文摘要:

Protein 4.1G is a member of the protein 4.1 family, which in general serves as adaptors linking transmembrane proteins to the cytoskeleton. 4.1G is thought to be widely expressed in many cells and tissues, but its function remains largely unknown. To explore the function of 4.1G in vivo, we generated 4.1G(-/-) mice and bred the mice in two backgrounds: C57BL/6 (B6) and 129/Sv (129) hybrids (B6-129) and inbred B6. Although the B6 4.1G(-/-) mice showed no obvious abnormalities, deficiency of 4.1G in B6-129 hybrids was associated with male infertility. Histological examinations of these 4.1G(-/-) mice revealed atrophy, impaired cell-cell contact and sloughing off of spermatogenic cells in seminiferous epithelium, and lack of mature spermatids in the epididymis. Ultrastructural examination revealed enlarged intercellular spaces between spermatogenic and Sertoli cells as well as the spermatid deformities. At the molecular level, 4.1G is associated with the nectin-like 4 (NECL4) adhesion molecule. Importantly, the expression of NECL4 was decreased, and the localization of NECL4 was altered in 4.1G(-/-) testis. Thus, our findings imply that 4.1G plays a role in spermatogenesis by mediating cell-cell adhesion between spermatogenic and Sertoli cells through its interaction with NECL4 on Sertoli cells. Additionally, the finding that infertility is present in B6-129 but not on the B6 background suggests the presence of a major modifier gene(s) that influences 4.1G function and is associated with male infertility.

语种: 英语
所属项目编号: DK 26263 ; HL31579 ; HL088468 ; DK32094
项目资助者: NIH
WOS记录号: WOS:000290475100009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54861
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
2.New York Blood Ctr, Mammalian Genet Lab, New York, NY 10065 USA
3.Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England
4.New York Blood Ctr, Lab Membrane Biol, New York, NY 10065 USA
5.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
6.Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA

Recommended Citation:
Yang, Shaomin,Weng, Haibo,Chen, Lixiang,et al. Lack of Protein 4.1G Causes Altered Expression and Localization of the Cell Adhesion Molecule Nectin-Like 4 in Testis and Can Cause Male Infertility[J]. MOLECULAR AND CELLULAR BIOLOGY,2011,31(11):2276-2286.
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