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Effects of Bone Marrow Mesenchymal Stem Cell Transplantation on Light-Damaged Retina
Zhang, Yu; Wang, Wei
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2010-07-01
DOI10.1167/iovs.08-3314
51期:7页:3742-3748
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]NEUROTROPHIC FACTOR GENE ; STROMAL CELLS ; IN-VITRO ; GROWTH-FACTOR ; CEREBRAL-ISCHEMIA ; CONSTANT LIGHT ; ADULT-RAT ; DIFFERENTIATION ; PHOTORECEPTORS ; RESCUE
英文摘要

PURPOSE. To investigate the effects and possible mechanisms of rat bone marrow mesenchymal stem cell (BMSC) transplantation on the light-damaged retinal structure and the apoptosis of photoreceptors.

METHODS. DAPI-labeled BMSCs were transplanted into the sub-retinal space of light-damaged Sprague-Dawley rats 10 days after exposure. BMSCs were cultivated with the supernatant of homogenized retina (SHR).

RESULTS. The outer nuclear layer (ONL) contained significantly more cells and the percentage of apoptotic ONL cells was significantly reduced in the BMSC transplantation group than in the phosphate-buffered solution injection group or the light damage group. Most DAPI-labeled BMSCs expressed brain-derived neurotrophic factor (BDNF). There was elevated basic fibroblast growth factor (bFGF) and BDNF immunoreactivity in the retinas of the BMSC transplantation group compared with the light damage group. In vitro culture showed that 10% of BMSCs changed from fusiform shape to multipolar shape. A fraction of cells expressed MAP2 or glial fibrillary acidic protein, and some cells expressed bFGF or BDNF when cultivated with light-damaged SHR for 7 days.

CONCLUSIONS. BMSC subretinal transplantation could inhibit photoreceptor apoptosis and slow down retinal damage in light-damaged eyes. BMSCs could express bFGF (in vitro) and BDNF (in vitro and in vivo), pointing to potential trophic and protective effects on light-damaged retinas. (Invest Ophthalmol Vis Sci. 2010;51:3742-3748) DOI:10.1167/iovs.08-3314

语种英语
WOS记录号WOS:000279047500056
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54862
专题北京大学第三临床医学院_眼科
作者单位Peking Univ, Hosp 3, Ctr Eye, Beijing 100191, Peoples R China
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Zhang, Yu,Wang, Wei. Effects of Bone Marrow Mesenchymal Stem Cell Transplantation on Light-Damaged Retina[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2010,51(7):3742-3748.
APA Zhang, Yu,&Wang, Wei.(2010).Effects of Bone Marrow Mesenchymal Stem Cell Transplantation on Light-Damaged Retina.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,51(7),3742-3748.
MLA Zhang, Yu,et al."Effects of Bone Marrow Mesenchymal Stem Cell Transplantation on Light-Damaged Retina".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 51.7(2010):3742-3748.
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