北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Effects of Bone Marrow Mesenchymal Stem Cell Transplantation on Light-Damaged Retina
作者: Zhang, Yu; Wang, Wei
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2010-07-01
DOI: 10.1167/iovs.08-3314
卷: 51, 期:7, 页:3742-3748
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: NEUROTROPHIC FACTOR GENE ; STROMAL CELLS ; IN-VITRO ; GROWTH-FACTOR ; CEREBRAL-ISCHEMIA ; CONSTANT LIGHT ; ADULT-RAT ; DIFFERENTIATION ; PHOTORECEPTORS ; RESCUE
英文摘要:

PURPOSE. To investigate the effects and possible mechanisms of rat bone marrow mesenchymal stem cell (BMSC) transplantation on the light-damaged retinal structure and the apoptosis of photoreceptors.

METHODS. DAPI-labeled BMSCs were transplanted into the sub-retinal space of light-damaged Sprague-Dawley rats 10 days after exposure. BMSCs were cultivated with the supernatant of homogenized retina (SHR).

RESULTS. The outer nuclear layer (ONL) contained significantly more cells and the percentage of apoptotic ONL cells was significantly reduced in the BMSC transplantation group than in the phosphate-buffered solution injection group or the light damage group. Most DAPI-labeled BMSCs expressed brain-derived neurotrophic factor (BDNF). There was elevated basic fibroblast growth factor (bFGF) and BDNF immunoreactivity in the retinas of the BMSC transplantation group compared with the light damage group. In vitro culture showed that 10% of BMSCs changed from fusiform shape to multipolar shape. A fraction of cells expressed MAP2 or glial fibrillary acidic protein, and some cells expressed bFGF or BDNF when cultivated with light-damaged SHR for 7 days.

CONCLUSIONS. BMSC subretinal transplantation could inhibit photoreceptor apoptosis and slow down retinal damage in light-damaged eyes. BMSCs could express bFGF (in vitro) and BDNF (in vitro and in vivo), pointing to potential trophic and protective effects on light-damaged retinas. (Invest Ophthalmol Vis Sci. 2010;51:3742-3748) DOI:10.1167/iovs.08-3314

语种: 英语
WOS记录号: WOS:000279047500056
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54862
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Hosp 3, Ctr Eye, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Yu,Wang, Wei. Effects of Bone Marrow Mesenchymal Stem Cell Transplantation on Light-Damaged Retina[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2010,51(7):3742-3748.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Yu]'s Articles
[Wang, Wei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Yu]‘s Articles
[Wang, Wei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace