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学科主题: 基础医学
题名:
Cerebralcare Granule (R) attenuates blood-brain barrier disruption after middle cerebral artery occlusion in rats
作者: Huang, Ping2; Zhou, Chang-Man3; Qin-Hu3; Liu, Yu-Ying2; Hu, Bai-He2; Chang, Xin2; Zhao, Xin-Rong2; Xu, Xiang-Shun2; Li, Quan2; Wei, Xiao-Hong2; Mao, Xiao-Wei1; Wang, Chuan-She1,2; Fan, Jing-Yu2; Han, Jing-Yan1,2
关键词: Blood-brain barrier ; Ischemic stroke ; Tight junction proteins ; Caveolin-1 ; Chinese medicine
刊名: EXPERIMENTAL NEUROLOGY
发表日期: 2012-10-01
DOI: 10.1016/j.expneurol.2012.07.017
卷: 237, 期:2, 页:453-463
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: ACUTE ISCHEMIC-STROKE ; DECOMPRESSIVE CRANIECTOMY ; INTRACRANIAL-PRESSURE ; FOCAL ISCHEMIA ; TIGHT JUNCTION ; IN-VIVO ; INJURY ; MICE ; REPERFUSION ; THROMBOLYSIS
英文摘要:

Disruption of blood-brain barrier (BBB) and subsequent edema are major contributors to the pathogenesis of ischemic stroke, for which the current clinical therapy remains unsatisfied. Cerebralcare Granule (R) (CG) is a compound Chinese medicine widely used in China for treatment of cerebrovascular diseases. CG has been demonstrated efficacy in attenuating the cerebral microcirculatory disturbance and hippocampal neuron injury following global cerebral ischemia. However, the effects of CG on BBB disruption following cerebral ischemia have not been investigated. In this study, we examined the therapeutic effect of CG on the BBB disruption in a focal cerebral ischemia/reperfusion (I/R) rat model. Male Sprague-Dawley rats (250 to 300 g) were subjected to 1 h middle cerebral artery occlusion (MCAO). CG (0.4 g/kg or 0.8 g/kg) was administrated orally 3 h after reperfusion for the first time and then once daily up to 6 days. The results showed that Evans blue extravasation, brain water content, albumin leakage, infarction volume and neurological deficits increased in MCAO model rats, and were attenuated significantly by CG treatment. T2-weighted MRI and electron microscopy further confirmed the brain edema reduction in CC-treated rats. Treatment with CG improved cerebral blood flow (CBF). Western blot analysis and confocal microscopy showed that the tight junction proteins claudin-5. JAM-1, occludin and zonula occluden-1 between endothelial cells were significantly degradated, but the protein expression of caveolin-1, the principal marker of caveolae in endothelial cells, increased after ischemia, all of which were alleviated by CG treatment. In conclusion, the post-treatment with CG significantly reduced BBB permeability and brain edema, which were correlated with preventing the degradation of the tight junction proteins and inhibiting the expression of caveolin-1 in the endothelial cells. These findings provide a novel approach to the treatment of ischemic stroke. (C) 2012 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 2008ZX09401 ; 20050230
项目资助者: Production of New Medicine Program of Ministry of Science and Technology of the People&prime ; s Republic of China ; Tianjin Tasly Croup, Tianjin, China
WOS记录号: WOS:000309034800023
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54869
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Integrat Chinese & Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Tasly Microcirculat Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Dept Anat, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Ping,Zhou, Chang-Man,Qin-Hu,et al. Cerebralcare Granule (R) attenuates blood-brain barrier disruption after middle cerebral artery occlusion in rats[J]. EXPERIMENTAL NEUROLOGY,2012,237(2):453-463.
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