IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Cerebralcare Granule (R) attenuates blood-brain barrier disruption after middle cerebral artery occlusion in rats
Huang, Ping2; Zhou, Chang-Man3; Qin-Hu3; Liu, Yu-Ying2; Hu, Bai-He2; Chang, Xin2; Zhao, Xin-Rong2; Xu, Xiang-Shun2; Li, Quan2; Wei, Xiao-Hong2; Mao, Xiao-Wei1; Wang, Chuan-She1,2; Fan, Jing-Yu2; Han, Jing-Yan1,2
关键词Blood-brain Barrier Ischemic Stroke Tight Junction Proteins Caveolin-1 Chinese Medicine
刊名EXPERIMENTAL NEUROLOGY
2012-10-01
DOI10.1016/j.expneurol.2012.07.017
237期:2页:453-463
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者Production of New Medicine Program of Ministry of Science and Technology of the People&prime ; s Republic of China ; Tianjin Tasly Croup, Tianjin, China ; Production of New Medicine Program of Ministry of Science and Technology of the People&prime ; s Republic of China ; Tianjin Tasly Croup, Tianjin, China
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]ACUTE ISCHEMIC-STROKE ; DECOMPRESSIVE CRANIECTOMY ; INTRACRANIAL-PRESSURE ; FOCAL ISCHEMIA ; TIGHT JUNCTION ; IN-VIVO ; INJURY ; MICE ; REPERFUSION ; THROMBOLYSIS
英文摘要

Disruption of blood-brain barrier (BBB) and subsequent edema are major contributors to the pathogenesis of ischemic stroke, for which the current clinical therapy remains unsatisfied. Cerebralcare Granule (R) (CG) is a compound Chinese medicine widely used in China for treatment of cerebrovascular diseases. CG has been demonstrated efficacy in attenuating the cerebral microcirculatory disturbance and hippocampal neuron injury following global cerebral ischemia. However, the effects of CG on BBB disruption following cerebral ischemia have not been investigated. In this study, we examined the therapeutic effect of CG on the BBB disruption in a focal cerebral ischemia/reperfusion (I/R) rat model. Male Sprague-Dawley rats (250 to 300 g) were subjected to 1 h middle cerebral artery occlusion (MCAO). CG (0.4 g/kg or 0.8 g/kg) was administrated orally 3 h after reperfusion for the first time and then once daily up to 6 days. The results showed that Evans blue extravasation, brain water content, albumin leakage, infarction volume and neurological deficits increased in MCAO model rats, and were attenuated significantly by CG treatment. T2-weighted MRI and electron microscopy further confirmed the brain edema reduction in CC-treated rats. Treatment with CG improved cerebral blood flow (CBF). Western blot analysis and confocal microscopy showed that the tight junction proteins claudin-5. JAM-1, occludin and zonula occluden-1 between endothelial cells were significantly degradated, but the protein expression of caveolin-1, the principal marker of caveolae in endothelial cells, increased after ischemia, all of which were alleviated by CG treatment. In conclusion, the post-treatment with CG significantly reduced BBB permeability and brain edema, which were correlated with preventing the degradation of the tight junction proteins and inhibiting the expression of caveolin-1 in the endothelial cells. These findings provide a novel approach to the treatment of ischemic stroke. (C) 2012 Elsevier Inc. All rights reserved.

语种英语
所属项目编号2008ZX09401 ; 20050230
资助者Production of New Medicine Program of Ministry of Science and Technology of the People&prime ; s Republic of China ; Tianjin Tasly Croup, Tianjin, China ; Production of New Medicine Program of Ministry of Science and Technology of the People&prime ; s Republic of China ; Tianjin Tasly Croup, Tianjin, China
WOS记录号WOS:000309034800023
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54869
Collection北京大学基础医学院
作者单位1.Peking Univ, Dept Integrat Chinese & Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Tasly Microcirculat Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Dept Anat, Sch Basic Med Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Huang, Ping,Zhou, Chang-Man,Qin-Hu,et al. Cerebralcare Granule (R) attenuates blood-brain barrier disruption after middle cerebral artery occlusion in rats[J]. EXPERIMENTAL NEUROLOGY,2012,237(2):453-463.
APA Huang, Ping.,Zhou, Chang-Man.,Qin-Hu.,Liu, Yu-Ying.,Hu, Bai-He.,...&Han, Jing-Yan.(2012).Cerebralcare Granule (R) attenuates blood-brain barrier disruption after middle cerebral artery occlusion in rats.EXPERIMENTAL NEUROLOGY,237(2),453-463.
MLA Huang, Ping,et al."Cerebralcare Granule (R) attenuates blood-brain barrier disruption after middle cerebral artery occlusion in rats".EXPERIMENTAL NEUROLOGY 237.2(2012):453-463.
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