IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科
学科主题临床医学
Osteopontin is involved in urotensin II-induced migration of rat aortic adventitial fibroblasts
Zhang, Yong-Gang1,2; Kuang, Ze-Jian1; Mao, Yan-Yan1; Wei, Rui-Hong3; Bao, Shi-Lin1; Wu, Li-Biao2; Li, Yu-Guang1; Tang, Chao-Shu4
关键词Osteopontin Urotensin Ii Migration Adventitial Fibroblasts Signal Transduction Peptide
刊名PEPTIDES
2011-12-01
DOI10.1016/j.peptides.2011.10.018
32期:12页:2452-2458
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]SMOOTH-MUSCLE-CELLS ; ANGIOTENSIN-II ; GROWTH-FACTOR ; ESSENTIAL-HYPERTENSION ; COLLAGEN-SYNTHESIS ; RECEPTOR BLOCKER ; CARDIAC FIBROSIS ; BALLOON INJURY ; KNOCKOUT MICE ; ATHEROSCLEROSIS
英文摘要

Recent studies suggest that both osteopontin and urotensin II (UII) play critical roles in vascular remodeling. We previously showed that UII could stimulate the migration of aortic adventitial fibroblasts. In this study, we examined whether osteopontin is involved in UII-induced migration of rat aortic adventitial fibroblasts and examined the effects and mechanisms of UII on osteopontin expression in adventitial fibroblasts. Migration of adventitial fibroblasts induced by UII could be inhibited significantly by osteopontin antisense oligonucleotide (P<0.01) but not sense or mismatch oligonucleotides (P>0.05). Moreover, UII dose- and time-dependently promoted osteopontin mRNA expression and protein secretion in the cells, with maximal effect at 10(-8) mol/l at 3h for mRNA expression or at 12h for protein secretion (both P<0.01). Furthermore, the UII effects were significantly inhibited by its receptor antagonist SB710411 (10(-6) mol/l), and Ca(2+) channel blocker nicardipine (10(-5) mol/l), protein kinase C (PKC) inhibitor H7 (10(-5) mol/l), calcineurin inhibitor cyclosporine A (10(-5) mol/l), mitogen-activated protein kinase (MAPK) inhibitor PD98059 (10(-5) mol/l) and Rho kinase inhibitor Y-27632 (10(-5) mol/l). Thus, osteopontin is involved in the UII-induced migration of adventitial fibroblasts, and UII could upregulate osteopontin gene expression and protein synthesis in rat aortic adventitial fibroblasts by activating its receptor and the Ca(2+) channel, PKC, calcineurin, MAPK and Rho kinase signal transduction pathways. (C) 2011 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000299194000010
项目编号30971273 ; 9151051501000016
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54870
专题北京大学第一临床医学院_心血管内科
作者单位1.Shantou Univ, Coll Med, Dept Cardiovasc Dis, Affiliated Hosp 1, Shantou 515041, Guangdong, Peoples R China
2.Shantou Univ, Coll Med, Mol Cardiol Lab, Affiliated Hosp 1, Shantou 515041, Guangdong, Peoples R China
3.Shantou Univ, Coll Med, Dept Internal Med, Affiliated Hosp 2, Shantou 515041, Guangdong, Peoples R China
4.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yong-Gang,Kuang, Ze-Jian,Mao, Yan-Yan,et al. Osteopontin is involved in urotensin II-induced migration of rat aortic adventitial fibroblasts[J]. PEPTIDES,2011,32(12):2452-2458.
APA Zhang, Yong-Gang.,Kuang, Ze-Jian.,Mao, Yan-Yan.,Wei, Rui-Hong.,Bao, Shi-Lin.,...&Tang, Chao-Shu.(2011).Osteopontin is involved in urotensin II-induced migration of rat aortic adventitial fibroblasts.PEPTIDES,32(12),2452-2458.
MLA Zhang, Yong-Gang,et al."Osteopontin is involved in urotensin II-induced migration of rat aortic adventitial fibroblasts".PEPTIDES 32.12(2011):2452-2458.
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