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学科主题: 临床医学
题名:
Osteopontin is involved in urotensin II-induced migration of rat aortic adventitial fibroblasts
作者: Zhang, Yong-Gang1,2; Kuang, Ze-Jian1; Mao, Yan-Yan1; Wei, Rui-Hong3; Bao, Shi-Lin1; Wu, Li-Biao2; Li, Yu-Guang1; Tang, Chao-Shu4
关键词: Osteopontin ; Urotensin II ; Migration ; Adventitial fibroblasts ; Signal transduction ; Peptide
刊名: PEPTIDES
发表日期: 2011-12-01
DOI: 10.1016/j.peptides.2011.10.018
卷: 32, 期:12, 页:2452-2458
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: SMOOTH-MUSCLE-CELLS ; ANGIOTENSIN-II ; GROWTH-FACTOR ; ESSENTIAL-HYPERTENSION ; COLLAGEN-SYNTHESIS ; RECEPTOR BLOCKER ; CARDIAC FIBROSIS ; BALLOON INJURY ; KNOCKOUT MICE ; ATHEROSCLEROSIS
英文摘要:

Recent studies suggest that both osteopontin and urotensin II (UII) play critical roles in vascular remodeling. We previously showed that UII could stimulate the migration of aortic adventitial fibroblasts. In this study, we examined whether osteopontin is involved in UII-induced migration of rat aortic adventitial fibroblasts and examined the effects and mechanisms of UII on osteopontin expression in adventitial fibroblasts. Migration of adventitial fibroblasts induced by UII could be inhibited significantly by osteopontin antisense oligonucleotide (P<0.01) but not sense or mismatch oligonucleotides (P>0.05). Moreover, UII dose- and time-dependently promoted osteopontin mRNA expression and protein secretion in the cells, with maximal effect at 10(-8) mol/l at 3h for mRNA expression or at 12h for protein secretion (both P<0.01). Furthermore, the UII effects were significantly inhibited by its receptor antagonist SB710411 (10(-6) mol/l), and Ca(2+) channel blocker nicardipine (10(-5) mol/l), protein kinase C (PKC) inhibitor H7 (10(-5) mol/l), calcineurin inhibitor cyclosporine A (10(-5) mol/l), mitogen-activated protein kinase (MAPK) inhibitor PD98059 (10(-5) mol/l) and Rho kinase inhibitor Y-27632 (10(-5) mol/l). Thus, osteopontin is involved in the UII-induced migration of adventitial fibroblasts, and UII could upregulate osteopontin gene expression and protein synthesis in rat aortic adventitial fibroblasts by activating its receptor and the Ca(2+) channel, PKC, calcineurin, MAPK and Rho kinase signal transduction pathways. (C) 2011 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30971273 ; 9151051501000016
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province of China
WOS记录号: WOS:000299194000010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54870
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Shantou Univ, Coll Med, Dept Cardiovasc Dis, Affiliated Hosp 1, Shantou 515041, Guangdong, Peoples R China
2.Shantou Univ, Coll Med, Mol Cardiol Lab, Affiliated Hosp 1, Shantou 515041, Guangdong, Peoples R China
3.Shantou Univ, Coll Med, Dept Internal Med, Affiliated Hosp 2, Shantou 515041, Guangdong, Peoples R China
4.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Yong-Gang,Kuang, Ze-Jian,Mao, Yan-Yan,et al. Osteopontin is involved in urotensin II-induced migration of rat aortic adventitial fibroblasts[J]. PEPTIDES,2011,32(12):2452-2458.
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