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学科主题: 药学
题名:
Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry
作者: Song, Yuelin1; Zhang, Na1,2,3; Jiang, Yong4; Li, Jun1; Zhao, Yunfang1; Shi, Shepo1; Tu, Pengfei1,4
刊名: RSC ADVANCES
发表日期: 2015
DOI: 10.1039/c4ra14088e
卷: 5, 期:9, 页:6419-6428
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: SHENFU INJECTION ; ENANTIOSPECIFIC DETERMINATION ; HYDROPHILIC METABOLITES ; PANAX-NOTOGINSENG ; CARDIAC-ARREST ; PORCINE MODEL ; LC-MS/MS ; COLUMN ; METABOLOMICS ; PRODUCTS
英文摘要:

Aconite alkaloids and ginsenosides have been demonstrated as the effective constituents in Shenfu injection (SFI), which is a widely used Chinese herbal formulation prepared from red ginseng and processed aconite root. Quality control is quite critical to meet the increasing demand of SFI in the market and to guarantee safety in clinics, in particular regarding the toxic aconite alkaloids. Given the significant merits of online solid phase extraction (SPE) for sample preparation, an online SPE-based method was developed to simultaneously quantify ten aconite alkaloids and thirteen ginsenosides in SFI using UHPLC-MS/MS. Because of their distinct ionization behaviors, polarity switching was implemented in the ion source domain with a scheduled program, and positive and negative ionization modes were applied for alkaloids and saponins, respectively. Quantitative terms of the proposed method with respect to linearity, limit of detection, lower limit of quantification, precision, and accuracy were evaluated, and the results indicated that the method is sensitive, convenient, and reliable. The developed method was successfully applied for the simultaneous determination of aconite alkaloids and ginsenosides in ten batches of SFI (SFI1-10). The validated method can be adopted as a meaningful tool for the quality control of SFI concerning aconite alkaloids and ginsenosides, and also it offers a reliable choice for the widely qualitative analysis of constituents in complex matrices being free from tedious sample preparation procedures.

语种: 英语
所属项目编号: 2013ZX09201018 ; 2012ZX09103201-036 ; 2012ZX09301002-002-002 ; 81403073
项目资助者: National Key Technology R&amp ; D Program "New Drug Innovation" of China ; National Natural Science Foundation of China
WOS记录号: WOS:000347410600026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54875
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Baotou Med Coll, Baotou 014060, Peoples R China
2.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China
3.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Song, Yuelin,Zhang, Na,Jiang, Yong,et al. Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry[J]. RSC ADVANCES,2015,5(9):6419-6428.
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