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Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry
Song, Yuelin1; Zhang, Na1,2,3; Jiang, Yong4; Li, Jun1; Zhao, Yunfang1; Shi, Shepo1; Tu, Pengfei1,4
刊名RSC ADVANCES
2015
DOI10.1039/c4ra14088e
5期:9页:6419-6428
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]SHENFU INJECTION ; ENANTIOSPECIFIC DETERMINATION ; HYDROPHILIC METABOLITES ; PANAX-NOTOGINSENG ; CARDIAC-ARREST ; PORCINE MODEL ; LC-MS/MS ; COLUMN ; METABOLOMICS ; PRODUCTS
英文摘要

Aconite alkaloids and ginsenosides have been demonstrated as the effective constituents in Shenfu injection (SFI), which is a widely used Chinese herbal formulation prepared from red ginseng and processed aconite root. Quality control is quite critical to meet the increasing demand of SFI in the market and to guarantee safety in clinics, in particular regarding the toxic aconite alkaloids. Given the significant merits of online solid phase extraction (SPE) for sample preparation, an online SPE-based method was developed to simultaneously quantify ten aconite alkaloids and thirteen ginsenosides in SFI using UHPLC-MS/MS. Because of their distinct ionization behaviors, polarity switching was implemented in the ion source domain with a scheduled program, and positive and negative ionization modes were applied for alkaloids and saponins, respectively. Quantitative terms of the proposed method with respect to linearity, limit of detection, lower limit of quantification, precision, and accuracy were evaluated, and the results indicated that the method is sensitive, convenient, and reliable. The developed method was successfully applied for the simultaneous determination of aconite alkaloids and ginsenosides in ten batches of SFI (SFI1-10). The validated method can be adopted as a meaningful tool for the quality control of SFI concerning aconite alkaloids and ginsenosides, and also it offers a reliable choice for the widely qualitative analysis of constituents in complex matrices being free from tedious sample preparation procedures.

语种英语
WOS记录号WOS:000347410600026
项目编号2013ZX09201018 ; 2012ZX09103201-036 ; 2012ZX09301002-002-002 ; 81403073
资助机构National Key Technology R&amp ; D Program "New Drug Innovation" of China ; National Natural Science Foundation of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54875
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学第三临床医学院_康复医学科
作者单位1.Baotou Med Coll, Baotou 014060, Peoples R China
2.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China
3.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Song, Yuelin,Zhang, Na,Jiang, Yong,et al. Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry[J]. RSC ADVANCES,2015,5(9):6419-6428.
APA Song, Yuelin.,Zhang, Na.,Jiang, Yong.,Li, Jun.,Zhao, Yunfang.,...&Tu, Pengfei.(2015).Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry.RSC ADVANCES,5(9),6419-6428.
MLA Song, Yuelin,et al."Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry".RSC ADVANCES 5.9(2015):6419-6428.
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