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学科主题临床医学
Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes
Fan, Yong1; Li, Rong2; Huang, Jin2; Yu, Yang2,3; Qiao, Jie2,3,4
关键词Triploid Zygotes Haploid Spindle Assembly Human Embryonic Stem Cells
刊名CELL CYCLE
2013-01-15
DOI10.4161/cc.23103
12期:2页:302-311
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]IN-VITRO FERTILIZATION ; MOUSE EMBRYOS ; CHROMOSOME-ABNORMALITIES ; PREIMPLANTATION EMBRYOS ; OXYGEN CONCENTRATIONS ; GROWTH-FACTOR ; HUMAN OOCYTES ; SELF-RENEWAL ; DERIVATION ; LINES
英文摘要

Human embryonic stem cells have shown tremendous potential in regenerative medicine, and the recent progress in haploid embryonic stem cells provides new insights for future applications of embryonic stem cells. Disruption of normal fertilized embryos remains controversial; thus, the development of a new source for human embryonic stem cells is important for their usefulness. Here, we investigated the feasibility of haploid and diploid embryo reconstruction and embryonic stem cell derivation using microsurgically repaired tripronuclear human zygotes. Diploid and haploid zygotes were successfully reconstructed, but a large proportion of them still had a tripolar spindle assembly. The reconstructed embryos developed to the blastocyst stage, although the loss of chromosomes was observed in these zygotes. Finally, triploid and diploid human embryonic stem cells were derived from tripronuclear and reconstructed zygotes (from which only one pronucleus was removed), but haploid human embryonic stem cells were not successfully derived from the reconstructed zygotes when two pronuclei were removed. Both triploid and diploid human embryonic stem cells showed the general characteristics of human embryonic stem cells. These results indicate that the lower embryo quality resulting from abnormal spindle assembly contributed to the failure of the haploid embryonic stem cell derivation. However, the successful derivation of diploid embryonic stem cells demonstrated that microsurgical tripronuclear zygotes are an alternative source of human embryonic stem cells. In the future, improving spindle assembly will facilitate the application of triploid zygotes to the field of haploid embryonic stem cells.

语种英语
WOS记录号WOS:000314601500020
项目编号2011CB944504 ; 30825038 ; 31000661 ; 81100473 ; U1132005 ; 2012J2200006 ; 2012KJCX0087
资助机构Ministry of Science and Technology of China Grants (973 program) ; Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China ; National Natural Science Funds for Young Scholar ; National Natural Science Foundation of China ; Zhujiang Science and Technology Star Project of Guangzhou ; Guangdong education fund
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54880
专题北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_生殖医学中心
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Guangzhou, Guangdong, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100871, Peoples R China
4.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Fan, Yong,Li, Rong,Huang, Jin,et al. Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes[J]. CELL CYCLE,2013,12(2):302-311.
APA Fan, Yong,Li, Rong,Huang, Jin,Yu, Yang,&Qiao, Jie.(2013).Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes.CELL CYCLE,12(2),302-311.
MLA Fan, Yong,et al."Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes".CELL CYCLE 12.2(2013):302-311.
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