IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device
Xie, Lan2,3; Ma, Rui2,3; Han, Chao2,3; Su, Kai1; Zhang, Qiufang4; Qiu, Tian2,3; Wang, Lei3; Huang, Guoliang2,3; Qiao, Jie4; Wang, Jundong1; Cheng, Jing2,3,5
刊名CLINICAL CHEMISTRY
2010-08-01
DOI10.1373/clinchem.2010.146902
56期:8页:1270-1278
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]MICROFLUIDIC SYSTEM ; CUMULUS CELLS ; SWIM-UP ; SPERMATOZOA ; SEPARATION ; MOUSE ; FERTILIZATION ; PROGESTERONE ; CAPACITATION ; COOPERATION
英文摘要

BACKGROUND: Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microdevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract.

METHODS: The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches.

RESULTS: The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies.

CONCLUSIONS: For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a lab-on-a-chip device to improve IVF outcome. (C) 2010 American Association for Clinical Chemistry

语种英语
WOS记录号WOS:000280501400015
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54884
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Natl Engn Res Ctr Beijing Biochip Technol, Beijing, Peoples R China
2.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China
3.Tsinghua Univ, Sch Med, Dept Biomed Engn, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100871, Peoples R China
5.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式
GB/T 7714
Xie, Lan,Ma, Rui,Han, Chao,et al. Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device[J]. CLINICAL CHEMISTRY,2010,56(8):1270-1278.
APA Xie, Lan.,Ma, Rui.,Han, Chao.,Su, Kai.,Zhang, Qiufang.,...&Cheng, Jing.(2010).Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device.CLINICAL CHEMISTRY,56(8),1270-1278.
MLA Xie, Lan,et al."Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device".CLINICAL CHEMISTRY 56.8(2010):1270-1278.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xie, Lan]的文章
[Ma, Rui]的文章
[Han, Chao]的文章
百度学术
百度学术中相似的文章
[Xie, Lan]的文章
[Ma, Rui]的文章
[Han, Chao]的文章
必应学术
必应学术中相似的文章
[Xie, Lan]的文章
[Ma, Rui]的文章
[Han, Chao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。