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学科主题: 临床医学
题名:
Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device
作者: Xie, Lan2,3; Ma, Rui2,3; Han, Chao2,3; Su, Kai1; Zhang, Qiufang4; Qiu, Tian2,3; Wang, Lei3; Huang, Guoliang2,3; Qiao, Jie4; Wang, Jundong1; Cheng, Jing2,3,5
刊名: CLINICAL CHEMISTRY
发表日期: 2010-08-01
DOI: 10.1373/clinchem.2010.146902
卷: 56, 期:8, 页:1270-1278
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: MICROFLUIDIC SYSTEM ; CUMULUS CELLS ; SWIM-UP ; SPERMATOZOA ; SEPARATION ; MOUSE ; FERTILIZATION ; PROGESTERONE ; CAPACITATION ; COOPERATION
英文摘要:

BACKGROUND: Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microdevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract.

METHODS: The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches.

RESULTS: The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies.

CONCLUSIONS: For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a lab-on-a-chip device to improve IVF outcome. (C) 2010 American Association for Clinical Chemistry

语种: 英语
WOS记录号: WOS:000280501400015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54884
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Natl Engn Res Ctr Beijing Biochip Technol, Beijing, Peoples R China
2.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China
3.Tsinghua Univ, Sch Med, Dept Biomed Engn, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100871, Peoples R China
5.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

Recommended Citation:
Xie, Lan,Ma, Rui,Han, Chao,et al. Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device[J]. CLINICAL CHEMISTRY,2010,56(8):1270-1278.
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