|Vasoactive effects of adrenotensin and its interactions with adrenomedullin|
|Zhou, L; Qiu, ZY; Ye, C; Di, L; Liu, XH; Tang, CS; Zhao, YT|
|关键词||Adrenotensin Adrenomedullin Vasoactive Effects Interaction|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
Objective To investigate the vasoactive effects of adrenotensin and the interactions between adrenotensin and adrenomedullin (ADM).
Methods isolated rat aortic tension, rat mean arterial pressure and H-3-TdR incorporation of rat vascular smooth muscle cells were measured. Isolated rat aortas were incubated in K-H solution containing adrenomedullin or adrenotensin. The released adrenotensin or adrenomedullin tin incubation medium) from rat aortas was measured by radioimmunoassay.
Results 1 x 10(-8) and 1 x 10(-7) mol/L adrenotensin augmented rat aortic tension in a dose-dependent manner (P < 0.01). An intravenous bolus injection of adrenotensin (2.5 nmol/kg, iv) increased the mean arterial pressure by 28% in anesthetized rats (P < 0.01). 1 x 10(-7)mol/L adrenotensin increased 3H-TdR incorporation in cultured rat vascular smooth muscle cells by 55% (P < 0.01). Adrenomedullin inhibited these activities of adrenotensin to different extents. 1 x 10(-9), 1 x 10(-8) and 1 x 10(-7) mol/L adrenotensin decreased adrenomedullin release rates by 19%, 35% and 46%, respectively (P < 0.05 or P < 0.01) and 1 x 10(-8)mol/L adrenomedullin also inhibited adrenotensin release by 45% from rat aorta ( P < 0.01).
Conclusion Adrenotensin is a novel peptide that elicits the activities of vasoconstriction, presser effects and induces the proliferation of vascular smooth muscle cells. There is antagonism in vascular activities and reciprocal inhibition in the release between adrenotensin and adrenomedullin. These interactions are manifestations of intramolecular regulation of proadrenomedullin (Pro-ADM).
|作者单位||1.Chongquing Med Univ, Ctr Lab, Dept Pathophysiol, Chongqing 400016, Peoples R China|
2.Beijing Med Univ, Inst Cardiovasc Res, Beijing 100083, Peoples R China
|Zhou, L,Qiu, ZY,Ye, C,et al. Vasoactive effects of adrenotensin and its interactions with adrenomedullin[J]. CHINESE MEDICAL JOURNAL,2000,113(3):269-271.|
|APA||Zhou, L.,Qiu, ZY.,Ye, C.,Di, L.,Liu, XH.,...&Zhao, YT.(2000).Vasoactive effects of adrenotensin and its interactions with adrenomedullin.CHINESE MEDICAL JOURNAL,113(3),269-271.|
|MLA||Zhou, L,et al."Vasoactive effects of adrenotensin and its interactions with adrenomedullin".CHINESE MEDICAL JOURNAL 113.3(2000):269-271.|