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学科主题: 临床医学
题名:
Long-term effects of neonatal seizures and exercise on learning, memory and CaMK II expression in hippocampus
作者: Ni Hong1; Jiang Yu-Wu2; Tao Lu-Yang1; Lou Jiang-Yan1; Wang Zhe-Dong1; Wu Xi-Ru2
关键词: seizure ; Morris water-maze ; physical exercise ; CaMK II
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2008
卷: 35, 期:1, 页:97-104
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: WISTAR RAT ; MECHANISMS
英文摘要:

Despite the clinical and experimental concerns about the deleterious effects of neonatal seizures on brain development, the underlying mechanism of seizure-induced brain damage is still not clear. Moreover, early therapeutic intervention studies are also less available. For this reason, the study was performed to explore the long-term effects of neonatal seizures and physical exercise on learning, memory and the expression of calcium/calmodulin- dependent protein kinase 11 (CaMK II). Twelve neonatal rats for each group were assigned: the single- seizure group (SS), the recurrent-seizure group (RS) and the control group. The volatile agent flurothyl was used to induce 30 min seizure attack. At postnatal day 6(P6), the single seizures induced only once and recurrent seizures induced once per day for consecutive 6 days. Control rats were placed into the container for an equal amount of time to their counterpart without exposure to flurothyl. Morris water-maze test were performed at P27 similar to P31, P58 similar to P61 and P80 similar to P82, meanwhile at P51 similar to P56, the RS and SS groups were submitted to forced running exercise. In situ hybridization method was used to detect the expression of CaMK 11 mRNA in hippocampus. The results are as follows: (1) Escape latency. In the first two Morris water-maze tests, there was a decreasing trend of escape latency in three groups, and the escape latency of RS group was much longer than that of control group. After physical exercise,in the last Morris water-maze tests, the diference of escape latency in three groups is not significant. (2) Searching strategy. In the first Morris water-maze test, there was a decreasing trend of marginal strategy and an increasing trend of taxis strategy in three groups, but the frequency of marginal strategy was higher and the frequency of taxis strategy was lower in RS group than that in SS and control group in the third and fourth day (P <0.01). However, there were no significant differences among three groups in the second and third Morris water-maze test. (3) Memory test. In the three memory tests, as for the distance ratioin between the origin platform quadrant to total distance, the RS group was the worst. The frequency of taxis strategy was much lower in RS group than that in SS and control groups in the first to third day(P < 0.01). (4) In situ hybridization detection showed that the expression of CaMK 11 mRNA in dentate gyrus and hilus was much lower in RS group than that in SS and control groups (P < 0.01). It was concluded that recurrent but not single prolonged seizures could cause long-term effects on learning and memory, which may be associated with the down-regulated expression of CaMK II mRNA in hippocampus. Physical exercise improved the learning capacity of RS group but with no effect on memory capacity of RS group.

语种: 中文
WOS记录号: WOS:000254046500014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54902
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Beijing 100034, Peoples R China
2.Suzhou Univ, Neurol Lab, Childrens Hosp, Lab Aging & Nervous Dis, Suzhou 215003, Peoples R China

Recommended Citation:
Ni Hong,Jiang Yu-Wu,Tao Lu-Yang,et al. Long-term effects of neonatal seizures and exercise on learning, memory and CaMK II expression in hippocampus[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2008,35(1):97-104.
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