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学科主题: 基础医学
题名:
Cloning of a novel human caspase-9 splice variant containing only the CARD domain
作者: Wang, Pingzhang; Shi, Taiping; Ma, Dalong
关键词: caspase-9 ; Casp9-gamma ; CARD ; NF-kappa B
刊名: LIFE SCIENCES
发表日期: 2006-08-01
DOI: 10.1016/j.lfs.2006.04.026
卷: 79, 期:10, 页:934-940
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: NF-KAPPA-B ; CELL-DEATH ; ACTIVATION ; APOPTOSIS ; INHIBITION ; PROTEIN ; FAMILY ; SITE
英文摘要:

Caspase-9 plays a key role in the intrinsic apoptotic pathway and currently two splice variants (caspase-9-alpha and -beta) have been identified. The present study cloned and characterized a novel caspase-9 splice variant, hereby designated Casp9-gamma. Casp9-gamma is generated from an additional alternative 3′ splice site in the fourth exon of caspase-9, resulting in a 58-nucleotide fragment insertion compared with the full-length caspase-9-alpha. The fragment introduces an in-frame stop codon, and the resulting open reading frame (ORE) is preterminated. The Casp9-gamma comprises the deduced 154 amino acid residues containing only the caspase recruitment domain (CARD) and does not contain the large and small subunits. The Casp9-gamma does not promote apoptosis when overexpressed in mammalian cells. Moreover, it inhibits the cleavage of procaspase-3 mediated by proapoptotic member Bax or apoptosis inductor staurosporine. Therefore, Casp9-gamma may function as an endogenous apoptotic inhibitor by interfering with the CARD-CARD interaction between Apaf-1 (apoptotic protease activating factor-1) and procaspase-9. In addition, Casp9-gamma does not enhance NF-kappa B activation in transfected 293T cells, conflicting with previous evidence that the isolated CARD of caspase-9 activates NF-kappa B in ND7 cells. This suggests that the procaspase-9-mediated NF-kappa B activation in response to cellular stresses is cell type-specific through an unidentified mechanism. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000239352600002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54931
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
3.Peking Univ, Human Dis Genom Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Wang, Pingzhang,Shi, Taiping,Ma, Dalong. Cloning of a novel human caspase-9 splice variant containing only the CARD domain[J]. LIFE SCIENCES,2006,79(10):934-940.
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