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学科主题临床医学
Effect of Acute, Slightly Increased Intra-Abdominal Pressure on Intestinal Permeability and Oxidative Stress in a Rat Model
Leng, Yuxin1; Zhang, Kuo2; Fan, Jie1; Yi, Min1; Ge, Qinggang1; Chen, Li3; Zhang, Lu4; Yao, Gaiqi1
刊名PLOS ONE
2014-10-08
DOI10.1371/journal.pone.0109350
9期:10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ABDOMINAL COMPARTMENT SYNDROME ; CRITICALLY-ILL PATIENTS ; BACTERIAL TRANSLOCATION ; INTERNATIONAL-CONFERENCE ; BLOOD-FLOW ; HYPERTENSION ; GUT ; INJURY ; ISCHEMIA ; HYPOPERFUSION
英文摘要

Introduction: Intra-abdominal hypertension (IAH) is known as a common, serious complication in critically ill patients. Bacterial translocation and permeability changes are considered the pathophysiological bases for IAH-induced enterogenic endotoxemia and subsequent multiorgan failure. Nevertheless, the effects of slightly elevated intra-abdominal pressures (IAPs) on the intestinal mucosa and the associated mechanisms remain unclear.

Methods: To investigate the acute effects of different nitrogen pneumoperitoneum grades on colonic mucosa, male Sprague-Dawley rats were assigned to six groups with different IAPs (0 [control], 4, 8, 12, 16, and 20 mmHg, n=6/group). During 90 min of exposure, we dynamically monitored the heart rate and noninvasive hemodynamic parameters. After gradual decompression, arterial blood gas analyses were conducted. Thereafter, structural injuries to the colonic mucosa were identified using light microscopy. Colon permeability was determined using the expression of tight junction proteins, combined with fluorescein isothiocyanate dextran (FD-4) absorption. The pro-oxidant-antioxidant balance was determined based on the levels of malondialdehyde (MDA) and antioxidant enzymes.

Results: IAH significantly affected the histological scores of the colonic mucosa, tight junction protein expression, mucosal permeability, and pro-oxidant-antioxidant balance. Interestingly, elevations of IAP that were lower than the threshold for IAH also showed a similar, undesirable effect. In the 8 mmHg group, mild hyponatremia, hypocalcemia, and hypoxemia occurred, accompanied by reduced blood and abdominal perfusion pressures. Mild microscopic inflammatory infiltration and increased MDA levels were also detected. Moreover, an 8-mm Hg IAP markedly inhibited the expression of tight junction proteins, although no significant differences in FD-4 permeability were observed between the 0- and 8-mmHg groups.

Conclusions: Acute exposure to slightly elevated IAP may result in adverse effects on intestinal permeability and the pro-oxidant-antioxidant balance. Therefore, in patients with critical illnesses, IAP should be dynamically monitored and corrected, as soon as possible, to prevent intestinal mucosal injury and subsequent gut-derived sepsis.

语种英语
WOS记录号WOS:000345204000063
项目编号Z141107002514020
资助机构Peking University - Tsinghua University Joint Center for Life Sciences ; Beijing science and technology plan
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54948
专题北京大学第三临床医学院_危重医学科
北京大学医学部管理机构_医学部
作者单位1.Peking Univ, Hosp 3, Dept Intens Care Unit, Beijing 100871, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Lab Anim Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 3, Dept Cent Lab, Beijing 100871, Peoples R China
4.Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Leng, Yuxin,Zhang, Kuo,Fan, Jie,et al. Effect of Acute, Slightly Increased Intra-Abdominal Pressure on Intestinal Permeability and Oxidative Stress in a Rat Model[J]. PLOS ONE,2014,9(10).
APA Leng, Yuxin.,Zhang, Kuo.,Fan, Jie.,Yi, Min.,Ge, Qinggang.,...&Yao, Gaiqi.(2014).Effect of Acute, Slightly Increased Intra-Abdominal Pressure on Intestinal Permeability and Oxidative Stress in a Rat Model.PLOS ONE,9(10).
MLA Leng, Yuxin,et al."Effect of Acute, Slightly Increased Intra-Abdominal Pressure on Intestinal Permeability and Oxidative Stress in a Rat Model".PLOS ONE 9.10(2014).
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