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学科主题临床医学
Molecular cloning, identification and analysis of lung squamous cell carcinoma-related genes
Shen, CY; Hui-Zhao; Wang, DL; Jiang, GC; Wang, J; Zhang, GL
关键词Suppression Subtractive Hybridization Lung Squamous Cell Carcinoma Clone Cdna
刊名LUNG CANCER
2002-12-01
38期:3页:235-241
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Respiratory System
研究领域[WOS]Oncology ; Respiratory System
关键词[WOS]PUTATIVE TUMOR-SUPPRESSOR ; EXPRESSION ; CARCINOGENESIS ; S100A2
英文摘要

Objective: To clone and identify genes differentially expressed in human lung squamous cell carcinoma (LSCC). Methods: A subtracted cDNA library of human LSCC was constructed by using suppression subtractive hybridization (SSH) method. Through screening, the subtracted library clones, representing mRNAs that are truly differentially expressed in LSCC but not in normal lung tissues, were selected out to identify by semi-quantitative RT-PCR in 12 patients of LSCC and performed DNA sequencing. Nucleic acid homology searches were performed using the BLAST program. Partial novel genes were detected by Northern blot. Results: By this technique, we obtained 10 differentially expressed gene cDNA fragments of LSCC. Among them six were already known genes; two sequences were already identified but their functions were still unknown (hypothetical protein); two were novel (GenBank accession number were AF363068 and AY032661, respectively). The results from semi-quantitative RT-PCR showed that the transcription expression level of these clones including PPP1CB, caluminin, S100A2, HSNOV1, OCIA and AY032661 was downregulated in 12 cases of LSCC, while the transcription of HSP90, ferritin, gp96 and AF363068 was up-regulated in same cases. Conclusion: SSH is a powerful technique of high sensitivity for the detection of differential gene expression in LSCC and an effective method to clone novel genes. Six already known genes identified by SSH technique have been already implicated in the pathogenesis of lung carcinogenesis, or they are involved in immunological defense mechanism in human body. Two hypothetical proteins probably also play an important role in lung cancer pathogenesis. The function of two novel genes in lung carcinogenesis is under research. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000180087500003
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54949
专题北京大学第二临床医学院_胸外科
作者单位Peking Univ, Peoples Hosp, Dept Thorac Surg, Beijing 100044, Peoples R China
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GB/T 7714
Shen, CY,Hui-Zhao,Wang, DL,et al. Molecular cloning, identification and analysis of lung squamous cell carcinoma-related genes[J]. LUNG CANCER,2002,38(3):235-241.
APA Shen, CY,Hui-Zhao,Wang, DL,Jiang, GC,Wang, J,&Zhang, GL.(2002).Molecular cloning, identification and analysis of lung squamous cell carcinoma-related genes.LUNG CANCER,38(3),235-241.
MLA Shen, CY,et al."Molecular cloning, identification and analysis of lung squamous cell carcinoma-related genes".LUNG CANCER 38.3(2002):235-241.
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