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学科主题: 公共卫生
题名:
Identification of tumor markers using two-dimensional electrophoresis in gastric carcinoma
作者: Wang, Kai-Juan2; Wang, Run-Tian1; Zhang, Jian-Zhong3
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-08-01
卷: 10, 期:15, 页:2179-2183
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: To study the differential expression of proteins in normal and cancerous gastric tissues, and further identify new molecular markers for diagnosis and prognosis of gastric carcinoma, as well as develop new therapeutic targets of the disease.

METHODS: Matched pairs of tissues from 6 gastric cancer patients were analyzed for their two-dimensional electrophoresis (2DE) profiles. Soluble fraction proteins from human normal and cancerous gastric tissue were separated in the first dimension by isoelectric focusing on immobilized pH gradient (IPG, pH3-10) strips, and by 125 g/L sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) in the second dimension with silver nitrate staining. Protein differential expession was analyzed by use of image analysis software to find out candidates for gastric cancer-associated proteins.

RESULTS: Nine protein spots overexpressed in tumor tissues as compared with noncancerous regions. In the next step, 9 tumor-specific spots were cut off from Coomassie Brilliant Blue staining gels, digested in gel with L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin. Protein identification was done by peptide mass fingerprinting with matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS).In total, 5 tumor-specific protein spots corresponding to 5 different polypeptide chains were identified, including annexin V, carbonic anhydrase, prohibitin, fibrin beta and fibrinogen fragment D. Among these 5 spots, the potential significance of the differential expressions is discussed.

CONCLUSION Differential expression analysis of proteomes may be useful for the development of new molecular markers for diagnosis and prognosis of gastric carcinoma.

Wang KJ, Wang RT, Zhang JZ. Identification of tumor markers using two-dimensional electrophoresis in gastric carcinoma. World J Gastroenterol 2004; 10(15): 2179-2183 http://www.wjgnet.com/1007-9327/10/2179.asp

语种: 英语
所属项目编号: 30070671
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000208317500005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54951
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Peking Univ, Sch Publ Hlth, Dept Epidemiol & Biostat, Beijing 100083, Peoples R China
2.Zhengzhou Univ, Sch Publ Hlth, Dept Epidemiol, Zhengzhou 450052, Henan Province, Peoples R China
3.Chines Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China

Recommended Citation:
Wang, Kai-Juan,Wang, Run-Tian,Zhang, Jian-Zhong. Identification of tumor markers using two-dimensional electrophoresis in gastric carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(15):2179-2183.
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