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学科主题: 基础医学
题名:
A model to induce low temperature trauma for in vitro astrogliosis study
作者: Yu, ACH; Wu, BY; Liu, RY; Li, Q; Li, YX; Wong, PF; Liu, S; Lau, LT; Fung, YWW
关键词: astrocytes ; glial fibrillary acidic protein (GFAP) ; gliosis ; heat shock protein ; immediate early gene ; injury model ; low temperature trauma
刊名: NEUROCHEMICAL RESEARCH
发表日期: 2004-11-01
卷: 29, 期:11, 页:2171-2176
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]: TRANSIENT COLD SHOCK ; GENE-EXPRESSION ; NEURONAL INJURY ; MESSENGER-RNA ; BRAIN-INJURY ; CEREBRAL-ISCHEMIA ; MECHANICAL INJURY ; C-FOS ; CULTURE ; PROTEIN
英文摘要:

Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care.

语种: 英语
WOS记录号: WOS:000225538200025
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54957
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Key Lab Neurosci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ PKU, Beijing 100871, Peoples R China
4.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
5.Chinese Acad Sci, Brain Res Inst, Shanghai, Peoples R China
6.Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai, Peoples R China
7.First Mil Med Univ, Nanfang Hosp, Res Ctr, Guangzhou, Peoples R China

Recommended Citation:
Yu, ACH,Wu, BY,Liu, RY,et al. A model to induce low temperature trauma for in vitro astrogliosis study[J]. NEUROCHEMICAL RESEARCH,2004,29(11):2171-2176.
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