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学科主题: 临床医学
题名:
Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer
作者: Xu, Zhiyun1; Gao, Yanghui1; Hao, Yuanyuan1; Li, Encheng1; Wang, Yan1; Zhang, Jianing2; Wang, Wenxin3; Gao, Zhancheng4,5; Wang, Qi1
关键词: 3D co-culture ; Individualized treatment ; Chemotherapy ; Microfluidic chip ; Drug sensitivity test ; Lung cancer
刊名: BIOMATERIALS
发表日期: 2013-05-01
DOI: 10.1016/j.biomaterials.2013.02.045
卷: 34, 期:16, 页:4109-4117
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: IN-VITRO ; STROMAL FIBROBLASTS ; CELLS ; RESISTANCE ; CHEMOTHERAPY ; ANGIOGENESIS ; PROGRESSION ; MODULATION ; SYSTEMS ; TUMORS
英文摘要:

Individualized treatment is a promising clinical strategy for lung cancer, and drug sensitivity testing is fundamental to this scheme. We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensional (3D) co-culture drug sensitivity test platform. A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo. The cells were treated with anticancer drugs according to a gradient concentration generator inside the chips to screen the appropriate chemotherapy schemes. We successfully cultured cell lines or primary cells with this device. We also smoothly assayed the sensitivities of different anti-cancer drugs in parallel and accurately screened appropriate-dose, single and combined-drug chemotherapy schemes for eight patients. Our microfluidic device is a simple, reliable, and high-throughput platform to test drug sensitivity. It would be possible for chemotherapists to screen the appropriate chemotherapy schemes to guide individualized treatment in lung cancer. (c) 2013 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2012CB822103 ; 91129733 ; 81071228
项目资助者: National High-tech R&amp ; D Program (973 Program) ; National Natural Science Foundation of China
WOS记录号: WOS:000317322600018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54968
Appears in Collections:北京大学第二临床医学院_呼吸内科_期刊论文

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作者单位: 1.Dalian Med Univ, Hosp 2, Dept Resp Med, Dalian 116027, Peoples R China
2.Dalian Med Univ, Inst Glycobiol, Dept Biochem, Dalian 116027, Peoples R China
3.Natl Univ Ireland, Network Excellence Funct Biomat, Galway, Ireland
4.Peking Univ, Peoples Hosp, Dept Resp Med, Beijing 100044, Peoples R China
5.Peking Univ, Peoples Hosp, Dept Crit Care Med, Beijing 100044, Peoples R China

Recommended Citation:
Xu, Zhiyun,Gao, Yanghui,Hao, Yuanyuan,et al. Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer[J]. BIOMATERIALS,2013,34(16):4109-4117.
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