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学科主题临床医学
Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer
Xu, Zhiyun1; Gao, Yanghui1; Hao, Yuanyuan1; Li, Encheng1; Wang, Yan1; Zhang, Jianing2; Wang, Wenxin3; Gao, Zhancheng4,5; Wang, Qi1
关键词3d Co-culture Individualized Treatment Chemotherapy Microfluidic Chip Drug Sensitivity Test Lung Cancer
刊名BIOMATERIALS
2013-05-01
DOI10.1016/j.biomaterials.2013.02.045
34期:16页:4109-4117
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]IN-VITRO ; STROMAL FIBROBLASTS ; CELLS ; RESISTANCE ; CHEMOTHERAPY ; ANGIOGENESIS ; PROGRESSION ; MODULATION ; SYSTEMS ; TUMORS
英文摘要

Individualized treatment is a promising clinical strategy for lung cancer, and drug sensitivity testing is fundamental to this scheme. We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensional (3D) co-culture drug sensitivity test platform. A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo. The cells were treated with anticancer drugs according to a gradient concentration generator inside the chips to screen the appropriate chemotherapy schemes. We successfully cultured cell lines or primary cells with this device. We also smoothly assayed the sensitivities of different anti-cancer drugs in parallel and accurately screened appropriate-dose, single and combined-drug chemotherapy schemes for eight patients. Our microfluidic device is a simple, reliable, and high-throughput platform to test drug sensitivity. It would be possible for chemotherapists to screen the appropriate chemotherapy schemes to guide individualized treatment in lung cancer. (c) 2013 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000317322600018
项目编号2012CB822103 ; 91129733 ; 81071228
资助机构National High-tech R&amp ; D Program (973 Program) ; National Natural Science Foundation of China
引用统计
被引频次:75[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54968
专题北京大学第二临床医学院_呼吸科
作者单位1.Dalian Med Univ, Hosp 2, Dept Resp Med, Dalian 116027, Peoples R China
2.Dalian Med Univ, Inst Glycobiol, Dept Biochem, Dalian 116027, Peoples R China
3.Natl Univ Ireland, Network Excellence Funct Biomat, Galway, Ireland
4.Peking Univ, Peoples Hosp, Dept Resp Med, Beijing 100044, Peoples R China
5.Peking Univ, Peoples Hosp, Dept Crit Care Med, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Xu, Zhiyun,Gao, Yanghui,Hao, Yuanyuan,et al. Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer[J]. BIOMATERIALS,2013,34(16):4109-4117.
APA Xu, Zhiyun.,Gao, Yanghui.,Hao, Yuanyuan.,Li, Encheng.,Wang, Yan.,...&Wang, Qi.(2013).Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer.BIOMATERIALS,34(16),4109-4117.
MLA Xu, Zhiyun,et al."Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer".BIOMATERIALS 34.16(2013):4109-4117.
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