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学科主题临床医学
EphA2 Targeted Doxorubicin Stealth Liposomes as a Therapy System for Choroidal Neovascularization in Rats
Wang, Jia-lin1; Liu, Yu-ling1; Li, Ying1; Dai, Wen-bing2; Guo, Zhao-ming2; Wang, Zhao-hui2; Zhang, Qiang2
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2012-10-01
DOI10.1167/iovs.12-9955
53期:11页:7348-7357
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者National Science Foundation ; Ministry of Science and Technology of China ; National Science Foundation ; Ministry of Science and Technology of China
研究领域[WOS]Ophthalmology
关键词[WOS]RECEPTOR TYROSINE KINASE ; INDUCED ANGIOGENESIS ; PHARMACOKINETICS ; MICROSPHERES ; ADRIAMYCIN ; DELIVERY ; TISSUES ; LIGAND ; GROWTH
英文摘要

PURPOSE. To enhance drug uptake in RPE cells, improve efficacy for choroidal neovascularization (CNV), and reduce drug toxicity, an EphA2-targeted nanocarrier loaded with doxorubicin (DOX) was developed by conjugation with a homing peptide YSA.

METHODS. The YSA was coupled to PEGylated lipid. Then, YSA-modified DOX stealth liposomes (YSA-SSL-DOX) were prepared and characterized. Their uptake in a human RPE cell line (ARPE-19) was evaluated. After intravitreous injection, their efficacy against CNV was assessed in a laser-induced rat model. Finally, TUNEL test and morphology observation on rat retina were conducted.

RESULTS. The prepared YSA-SSL-DOX was approximately 110 nm in particle size, with an encapsulation efficiency of DOX more than 95%. The leakage of DOX from YSA-SSL-DOX was very slow. The expression of EphA2 on the CNV was confirmed. Both flow cytometry and confocal microscopy studies revealed that YSA-SSL-DOX could facilitate the uptake of liposomal DOX into ARPE-19 cells. Treatment with YSA-SSL-DOX (2.5 mu g DOX) resulted in a significant reduction in the CNV area of rats compared with the unmodified liposomal DOX and normal saline (P < 0.05). No obvious toxicity of YSA-SSL-DOX on rat retina was found.

CONCLUSIONS. EphA2-targeted stealth liposomes might be an effective delivery and therapy system for angiogenesis-related diseases in the retina. (Invest Ophthalmol Vis Sci. 2012; 53: 7348-7357) DOI:10.1167/iovs.12-9955

语种英语
所属项目编号81130059 ; 2009CB930300 ; 2009ZX09310-001
资助者National Science Foundation ; Ministry of Science and Technology of China ; National Science Foundation ; Ministry of Science and Technology of China
WOS记录号WOS:000310589900085
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54991
Collection北京大学第三临床医学院_眼科
作者单位1.Peking Univ, Hosp 3, Ctr Eye, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jia-lin,Liu, Yu-ling,Li, Ying,et al. EphA2 Targeted Doxorubicin Stealth Liposomes as a Therapy System for Choroidal Neovascularization in Rats[J]. INVESTIGATIVE OPHTHALMOLOGY &amp; VISUAL SCIENCE,2012,53(11):7348-7357.
APA Wang, Jia-lin.,Liu, Yu-ling.,Li, Ying.,Dai, Wen-bing.,Guo, Zhao-ming.,...&Zhang, Qiang.(2012).EphA2 Targeted Doxorubicin Stealth Liposomes as a Therapy System for Choroidal Neovascularization in Rats.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,53(11),7348-7357.
MLA Wang, Jia-lin,et al."EphA2 Targeted Doxorubicin Stealth Liposomes as a Therapy System for Choroidal Neovascularization in Rats".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 53.11(2012):7348-7357.
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