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学科主题: 基础医学
题名:
Requirement of the NF-kappa B pathway for induction of Wnt-5A by interleukin-1 beta in condylar chondrocytes of the temporomandibular joint: functional crosstalk between the Wnt-5A and NF-kappa B signaling pathways
作者: Xian-Peng, Ge1; Can, Ye-Hua1; Zhang, Chen-Guang2; Zhou, Chun-Yan2; Ma, Kang-Tao2; Meng, Juan-Hong1; Ma, Xu-Chen1
关键词: Wnt-5A ; NF-kappa B ; Interleukin-1 beta ; Chondrocyte ; Temporomandibular joint
刊名: OSTEOARTHRITIS AND CARTILAGE
发表日期: 2011
DOI: 10.1016/j.joca.2010.10.016
卷: 19, 期:1, 页:111-117
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Orthopedics ; Rheumatology
研究领域[WOS]: Orthopedics ; Rheumatology
关键词[WOS]: HUMAN ARTICULAR CHONDROCYTES ; NITRIC-OXIDE SYNTHASE ; BETA-CATENIN ; RHEUMATOID-ARTHRITIS ; GENE-EXPRESSION ; OSTEOARTHRITIS ; ACTIVATION ; WNT5A ; CELLS ; METALLOPROTEINASE
英文摘要:

Objective: We have previously reported that interleukin-1 beta (IL-1 beta) up-regulates the expression of Wnt-5A and the activation of Wnt-5A signaling induces matrix metalloproteinase (MMP) through the c-Jun N-terminal kinase pathway in condylar chondrocytes (CCs) of the temporomandibular joint (TMJ). These results suggest that Wnt-5A could play an essential role in IL-1 beta-mediated cartilage destruction. The objective of this study was to investigate the molecular mechanism underlying IL-1 beta-induced up-regulation of Wnt-5A in TMJ CCs.

Methods: Primary CCs, limb chondrocytes (LCs) and SW1353 human chondrosarcoma cells were treated with IL-1 beta in the presence or absent of BAY 11-7082 (an inhibitor of I kappa B alpha-phosphorylation). Then, expression of Wnt-5A was estimated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Western blotting and immunocytofluorescence. Transient transfection of p65 expression vector and chromatin immunoprecipitation (ChIP) assay was performed to define the effect of p65 on Wnt-5A expression.

Results: IL-1 beta up-regulated Wnt-5A expression at both the RNA and protein levels in articular chondrocytes. The inhibitor of I kappa B alpha-phosphorylation, BAY 11-7082, blocked the induction of Wnt-5A by IL-1 beta in a dose-dependent manner. Moreover, experiments with overexpression of p65 and ChIP established that induction of Wnt-5A by IL-1 beta is mediated through the NF-kappa B pathway, especially the p65 subunit.

Conclusion: These results clarify the molecular mechanism underlying up-regulation of Wnt-5A by IL-1 beta in chondrocytes, suggesting an important functional crosstalk between Wnt-5A and NF-kappa B signaling pathways. This finding provides new insights into the involvement of Wnt signaling in the cartilage destruction caused by arthritis. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30772439
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000286542300014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55011
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100081, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China

Recommended Citation:
Xian-Peng, Ge,Can, Ye-Hua,Zhang, Chen-Guang,et al. Requirement of the NF-kappa B pathway for induction of Wnt-5A by interleukin-1 beta in condylar chondrocytes of the temporomandibular joint: functional crosstalk between the Wnt-5A and NF-kappa B signaling pathways[J]. OSTEOARTHRITIS AND CARTILAGE,2011,19(1):111-117.
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