IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Requirement of the NF-kappa B pathway for induction of Wnt-5A by interleukin-1 beta in condylar chondrocytes of the temporomandibular joint: functional crosstalk between the Wnt-5A and NF-kappa B signaling pathways
Xian-Peng, Ge1; Can, Ye-Hua1; Zhang, Chen-Guang2; Zhou, Chun-Yan2; Ma, Kang-Tao2; Meng, Juan-Hong1; Ma, Xu-Chen1
关键词Wnt-5a Nf-kappa b Interleukin-1 Beta Chondrocyte Temporomandibular Joint
刊名OSTEOARTHRITIS AND CARTILAGE
2011
DOI10.1016/j.joca.2010.10.016
19期:1页:111-117
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Rheumatology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Orthopedics ; Rheumatology
关键词[WOS]HUMAN ARTICULAR CHONDROCYTES ; NITRIC-OXIDE SYNTHASE ; BETA-CATENIN ; RHEUMATOID-ARTHRITIS ; GENE-EXPRESSION ; OSTEOARTHRITIS ; ACTIVATION ; WNT5A ; CELLS ; METALLOPROTEINASE
英文摘要

Objective: We have previously reported that interleukin-1 beta (IL-1 beta) up-regulates the expression of Wnt-5A and the activation of Wnt-5A signaling induces matrix metalloproteinase (MMP) through the c-Jun N-terminal kinase pathway in condylar chondrocytes (CCs) of the temporomandibular joint (TMJ). These results suggest that Wnt-5A could play an essential role in IL-1 beta-mediated cartilage destruction. The objective of this study was to investigate the molecular mechanism underlying IL-1 beta-induced up-regulation of Wnt-5A in TMJ CCs.

Methods: Primary CCs, limb chondrocytes (LCs) and SW1353 human chondrosarcoma cells were treated with IL-1 beta in the presence or absent of BAY 11-7082 (an inhibitor of I kappa B alpha-phosphorylation). Then, expression of Wnt-5A was estimated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Western blotting and immunocytofluorescence. Transient transfection of p65 expression vector and chromatin immunoprecipitation (ChIP) assay was performed to define the effect of p65 on Wnt-5A expression.

Results: IL-1 beta up-regulated Wnt-5A expression at both the RNA and protein levels in articular chondrocytes. The inhibitor of I kappa B alpha-phosphorylation, BAY 11-7082, blocked the induction of Wnt-5A by IL-1 beta in a dose-dependent manner. Moreover, experiments with overexpression of p65 and ChIP established that induction of Wnt-5A by IL-1 beta is mediated through the NF-kappa B pathway, especially the p65 subunit.

Conclusion: These results clarify the molecular mechanism underlying up-regulation of Wnt-5A by IL-1 beta in chondrocytes, suggesting an important functional crosstalk between Wnt-5A and NF-kappa B signaling pathways. This finding provides new insights into the involvement of Wnt signaling in the cartilage destruction caused by arthritis. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号30772439
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000286542300014
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55011
专题北京大学基础医学院
作者单位1.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100081, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xian-Peng, Ge,Can, Ye-Hua,Zhang, Chen-Guang,et al. Requirement of the NF-kappa B pathway for induction of Wnt-5A by interleukin-1 beta in condylar chondrocytes of the temporomandibular joint: functional crosstalk between the Wnt-5A and NF-kappa B signaling pathways[J]. OSTEOARTHRITIS AND CARTILAGE,2011,19(1):111-117.
APA Xian-Peng, Ge.,Can, Ye-Hua.,Zhang, Chen-Guang.,Zhou, Chun-Yan.,Ma, Kang-Tao.,...&Ma, Xu-Chen.(2011).Requirement of the NF-kappa B pathway for induction of Wnt-5A by interleukin-1 beta in condylar chondrocytes of the temporomandibular joint: functional crosstalk between the Wnt-5A and NF-kappa B signaling pathways.OSTEOARTHRITIS AND CARTILAGE,19(1),111-117.
MLA Xian-Peng, Ge,et al."Requirement of the NF-kappa B pathway for induction of Wnt-5A by interleukin-1 beta in condylar chondrocytes of the temporomandibular joint: functional crosstalk between the Wnt-5A and NF-kappa B signaling pathways".OSTEOARTHRITIS AND CARTILAGE 19.1(2011):111-117.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xian-Peng, Ge]的文章
[Can, Ye-Hua]的文章
[Zhang, Chen-Guang]的文章
百度学术
百度学术中相似的文章
[Xian-Peng, Ge]的文章
[Can, Ye-Hua]的文章
[Zhang, Chen-Guang]的文章
必应学术
必应学术中相似的文章
[Xian-Peng, Ge]的文章
[Can, Ye-Hua]的文章
[Zhang, Chen-Guang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。