北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Intermedin inhibits vascular calcification by increasing the level of matrix gamma-carboxyglutamic acid protein
作者: Cai, Yan1; Xu, Ming-Jiang1; Teng, Xu1; Zhou, Ye Bo1; Chen, Li2; Zhu, Yi1,2; Wang, Xian1,2; Tang, Chao Shu1,2; Qi, Yong Fen1,2,3
关键词: Vascular calcification ; Peptides ; Matrix gamma-carboxyglutamic (Gla) protein ; Phenotype transition
刊名: CARDIOVASCULAR RESEARCH
发表日期: 2010-03-01
DOI: 10.1093/cvr/cvp366
卷: 85, 期:4, 页:864-873
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: SMOOTH-MUSCLE-CELLS ; BONE MORPHOGENETIC PROTEIN-2 ; VITAMIN-D-3 PLUS NICOTINE ; HORMONE-RELATED PEPTIDE ; CHRONIC KIDNEY-DISEASE ; GLA PROTEIN ; ARTERIAL CALCIFICATION ; IN-VITRO ; PARATHYROID-HORMONE ; PROTECTIVE FACTOR
英文摘要:

Vascular calcification (VC) is highly associated with increased morbidity and mortality in patients with advanced chronic kidney disease. Paracrine/autocrine factors such as vasoactive peptides are involved in VC development. Here, we investigated the expression of the novel peptide intermedin (IMD) in the vasculature, tested its ability to prevent VC in vivo and in vitro, and examined the mechanism involved.

Rat VC was induced by administration of vitamin D-3 plus nicotine (VDN). IMD (100 ng kg(-1) h(-1)) was systemically administered by a mini-osmotic pump. VDN-treated rat aortas showed lower IMD content and increased expression of its receptors, along with increased vascular calcium deposition and alkaline phosphatase (ALP) activity. Low IMD levels were accompanied by increased calcium deposition in human atherosclerotic plaques. In vivo administration of IMD greatly reduced vascular calcium deposition and ALP activity in VDN-treated rats when compared with vehicle treatment, which was further confirmed in cultured vascular smooth muscle cells. Concurrently, the loss of smooth-muscle lineage markers and matrix gamma-carboxyglutamic acid (Gla) protein (cMGP) in aortas was ameliorated by administering IMD to rats with VC, and the increased phosphor-Smad(1/5/8) and core binding factor alpha-1 levels in calcified vasculature were also reduced. However, the inhibitory effects of IMD on VC were eliminated upon pre-treatment with warfarin or small interfering RNA to reduce cMGP.

Reduced endogenous IMD levels are associated with increased mineralization in vivo, and administration of IMD inhibits VC development by increasing cMGP levels. IMD may be an endogenous vasoprotective factor for VC.

语种: 英语
所属项目编号: 30470693 ; 30770869 ; 30871013 ; 2006CB503807
项目资助者: National Natural Science Foundation of China ; State Major Basic Research Development Program of the People&prime ; s Republic of China
WOS记录号: WOS:000274487900026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55017
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Physiol & Pathophysiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Minist Educ Res, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Lab Cardiovasc Bioact Mol, Beijing 100191, Peoples R China

Recommended Citation:
Cai, Yan,Xu, Ming-Jiang,Teng, Xu,et al. Intermedin inhibits vascular calcification by increasing the level of matrix gamma-carboxyglutamic acid protein[J]. CARDIOVASCULAR RESEARCH,2010,85(4):864-873.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Cai, Yan]'s Articles
[Xu, Ming-Jiang]'s Articles
[Teng, Xu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Cai, Yan]‘s Articles
[Xu, Ming-Jiang]‘s Articles
[Teng, Xu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace