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学科主题: 临床医学
题名:
PERP gene therapy attenuates lung cancer xenograft via inducing apoptosis and suppressing VEGF
作者: Chen, Ke1,2,3,4; Luo, Zhaoyang4; Li, Zhongwu5; Liu, Yiqiang5; Zhao, Qingzheng2,3
关键词: PERP ; gene therapy ; lung cancer ; apoptosis ; VEGF ; Caspase-3 ; Smac ; TRAIL
刊名: CANCER BIOLOGY & THERAPY
发表日期: 2011-12-15
DOI: 10.4161/cbt.13.1.18435
卷: 12, 期:12, 页:1114-1119
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: P53-DEPENDENT APOPTOSIS ; P53 ; HETEROZYGOSITY ; CHROMOSOME-6 ; MELANOMA ; MEDIATOR ; IMPROVES ; FAMILY ; THW
英文摘要:

Inducing apoptosis is an attractive antitumor strategy. PERP is an apoptosis-associated target of p53, and its activation alone is sufficient to induce apoptotic pathway leading to cell death. We have previously demonstrated that overexpression of PERP in tumor cell lines with low intrinsic PERP activity suppressed cancer cell growth and enhanced sensitivity to chemotherapeutical agents. We further identified that PERP was present in surgical normal lung tissue, but absent in cancerous tissue of the same patient. Here, we sought to investigate the anti-tumor effects of PERP gene therapy in vivo. Then nude mice were transplanted with p53-mutanted Anip973 human lung cancer xenografts and treated with normal saline, pcDNA3.1 (vector) and pcDNA3.1-PERP, respectively. Successful transfection and robust expression of PERP was detected. Treatment with pcDNA3.1-PERP increased apoptosis and retarded growth in the xenografts, which contributed to a 55% decrease in tumor volume compared with controls. Furthermore, PERP gene therapy activated pro-apoptotic Caspase-3 cascade and upregulated the expression of the second mitochondria-derived activator of caspase (Smac) and human TNF-related apoptosis-inducing ligand (TRAIL), while suppressed vascular endothelial growth factor (VEGF) expression, indicating apoptosis and anti-angiogenesis are involved in the inhibitory effect of the PERP gene therapy. Taken together, our results suggest PERP gene therapy may supply an alternative strategy for lung adenocarcinoma management. Furthermore, Anip973 is a p53-mutanted cell line and the findings of this study provide reference value for other p53-mutanted cancers which is common among malignant tumors.

语种: 英语
所属项目编号: 81101940
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000299629600013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55019
Appears in Collections:北京大学临床肿瘤学院_病理科_期刊论文

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作者单位: 1.Univ S China, Inst Canc Res, Hengyang City, Peoples R China
2.Chinese Acad Sci, Lab Dis Genom & Personalized Med, Beijing Inst Genom, Beijing, Peoples R China
3.Chinese Acad Med Sci, Dept Cellular & Mol Biol, Canc Inst & Hosp, Beijing 100730, Peoples R China
4.Peking Union Med Coll, Beijing 100021, Peoples R China
5.Peking Univ, Dept Pathol, Beijing Canc Hosp & Inst, Sch Oncol, Beijing 100871, Peoples R China

Recommended Citation:
Chen, Ke,Luo, Zhaoyang,Li, Zhongwu,et al. PERP gene therapy attenuates lung cancer xenograft via inducing apoptosis and suppressing VEGF[J]. CANCER BIOLOGY & THERAPY,2011,12(12):1114-1119.
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