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Studies on the nuclease activity and interactions of the mixed-polypyridyl [Ni-2(1,3-tpbd)(diimine)(2)(H2O)(2)](4+) complexes with thioredoxin reductase
Li, Dong-Dong1,2; Zeng, Hui-Hui1,2
关键词Mixed-polypyridyl Complexes Nuclease Activity Thioredoxin Reductase Inhibitors
刊名APPLIED ORGANOMETALLIC CHEMISTRY
2013-02-01
DOI10.1002/aoc.2945
27期:2页:89-97
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Applied ; Chemistry, Inorganic & Nuclear
研究领域[WOS]Chemistry
关键词[WOS]CELL GROWTH-INHIBITION ; ORGANOTELLURIUM COMPOUNDS ; COPPER(II) COMPLEXES ; ANTITUMOR AGENTS ; NICKEL-COMPLEXES ; DNA OXIDATION ; BINDING ; FLUORESCENCE ; SELECTIVITY ; ETHIDIUM
英文摘要

Three new nickel(II) complexes formulated as [Ni2(1,3-tpbd)(diimine)2(H2O)2]4+ [1,3-tpbd?=?N,N,N′,N′-tetrakis(2-pyridylmethyl)benzene-1,3-diamine, where diimine is an N,N-donor heterocyclic base like 1,10-phenanthroline (phen),2,2′-bipyridine (bpy), 4,5-diazafluoren-9-one (dafo)], have been synthesized and structurally characterized by X-ray crystallography: [Ni2(1,3-tpbd)(phen)2(H2O)2]4+ (1), [Ni2(1,3-tpbd)(bpy)2(H2O)2]4+(2) and [Ni2(1,3-tpbd)(dafo)2(H2O)2]4+ (3). Single-crystal diffraction reveals that the metal atoms in the complexes are all in a distorted octahedral geometry and in a trans arrangement around 1,3-tpbd ligand. The interactions of the three complexes with calf thymus DNA (CT-DNA) have been investigated by UV absorption, fluorescence spectroscopy, circular dichroism and viscosity. The apparent binding constant (Kapp) values are calculated to be 1.91 x 105? m-1 for 1, 1.18 x 105? m-1 for 2, and 1.35 x105? m-1 for 3, following the order 1?>?3?>?2. The higher DNA binding affinity of 1 is due to the involvement in partial insertion of the phen ring between the DNA base pairs. A decrease in relative viscosities of DNA upon binding to 13 is consistent with the DNA binding affinities. These complexes efficiently display oxidative cleavage of supercoiled DNA in the presence of H2O2 (250?mu m), with 3 exhibiting the highest nuclease activity. The rate constants for the conversion of supercoiled to nicked DNA are 5.28 x 10-5?s-1 (for 1), 6.67 x 10-5?s-1 (for 2) and 1.39 x 10-4?s-1 (for 3), also indicating that complex 3 shows higher catalytic activity than 1 and 2. Here the nuclease activity is not readily correlated to binding affinity. The inhibitory effect of complexes 13 on thioredoxin reductase has also been examined. The IC50 values are calculated to be 26.54?+/-?0.57, 31.03?+/-?3.33 and 8.69?+/-?2.54?mu m, respectively, showing a more marked inhibitory effect on thioredoxin reductase by complex 3 than the other two complexes. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000313890200006
项目编号2011M500526
资助机构China Postdoctoral Science Foundation
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55037
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Tianjin Int Joint Acad Biotechnol & Med, Tianjin 300457, Peoples R China
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Li, Dong-Dong,Zeng, Hui-Hui. Studies on the nuclease activity and interactions of the mixed-polypyridyl [Ni-2(1,3-tpbd)(diimine)(2)(H2O)(2)](4+) complexes with thioredoxin reductase[J]. APPLIED ORGANOMETALLIC CHEMISTRY,2013,27(2):89-97.
APA Li, Dong-Dong,&Zeng, Hui-Hui.(2013).Studies on the nuclease activity and interactions of the mixed-polypyridyl [Ni-2(1,3-tpbd)(diimine)(2)(H2O)(2)](4+) complexes with thioredoxin reductase.APPLIED ORGANOMETALLIC CHEMISTRY,27(2),89-97.
MLA Li, Dong-Dong,et al."Studies on the nuclease activity and interactions of the mixed-polypyridyl [Ni-2(1,3-tpbd)(diimine)(2)(H2O)(2)](4+) complexes with thioredoxin reductase".APPLIED ORGANOMETALLIC CHEMISTRY 27.2(2013):89-97.
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